{"id":5098,"date":"2025-09-11T11:43:11","date_gmt":"2025-09-11T03:43:11","guid":{"rendered":"https:\/\/www.dxfluidics.com\/?p=5098"},"modified":"2025-09-11T11:43:14","modified_gmt":"2025-09-11T03:43:14","slug":"%e5%be%ae%e6%b5%81%e6%8e%a7%e6%8a%80%e6%9c%af%e5%88%b6%e5%a4%87%e7%ba%bf%e7%b2%92%e4%bd%93%e6%9d%82%e5%90%88%e7%bb%86%e8%83%9e%e5%8f%8a%e4%b8%b4%e5%ba%8a%e5%ba%94%e7%94%a8%e5%88%9b%e6%96%b0","status":"publish","type":"post","link":"https:\/\/www.dxfluidics.com\/en\/technical-support\/information\/5098\/%e5%be%ae%e6%b5%81%e6%8e%a7%e6%8a%80%e6%9c%af%e5%88%b6%e5%a4%87%e7%ba%bf%e7%b2%92%e4%bd%93%e6%9d%82%e5%90%88%e7%bb%86%e8%83%9e%e5%8f%8a%e4%b8%b4%e5%ba%8a%e5%ba%94%e7%94%a8%e5%88%9b%e6%96%b0\/","title":{"rendered":"\u5fae\u6d41\u63a7\u6280\u672f\u9a71\u52a8\u7ebf\u7c92\u4f53\u6742\u5408\u7ec6\u80de\u5236\u5907\uff1a\u4ece\u82af\u7247\u52a0\u5de5\u5230\u4e34\u5e8a\u5e94\u7528\u7684\u521b\u65b0\u8def\u5f84"},"content":{"rendered":"<p class=\"wp-block-paragraph\">\u5728\u7ec6\u80de\u751f\u7269\u5b66\u4e0e\u5fae\u7eb3\u5236\u9020\u4ea4\u53c9\u9886\u57df\uff0c\u7ebf\u7c92\u4f53\u6742\u5408\u7ec6\u80de\uff08transmitochondrial\u00a0cybrids\uff09\u7684\u5236\u5907\u6280\u672f\u6b63\u7ecf\u5386\u4ece\u4f20\u7edf\u65b9\u6cd5\u5230\u5fae\u6d41\u63a7\u82af\u7247\u6280\u672f\u7684\u9769\u65b0\u3002\u4f20\u7edf\u65b9\u6848\u4f9d\u8d56\u65e0\u4e19\u916e\u9178\u00a0\/\u00a0\u5c3f\u82f7\uff08PU\uff09\u57f9\u517b\u57fa\u8fdb\u884c\u9009\u62e9\u6027\u57f9\u517b\uff0c\u800c\u57fa\u4e8e\u5fae\u6d41\u63a7\u88c5\u7f6e\u7684\u65b0\u65b9\u6cd5\u7a81\u7834\u4e86\u8fd9\u4e00\u9650\u5236\uff0c\u5b9e\u73b0\u4e86\u00a0PU\u00a0\u8865\u5145\u6761\u4ef6\u4e0b\u7684\u9ad8\u6548\u5236\u5907\u3002\u672c\u6587\u5c06\u7cfb\u7edf\u89e3\u6790\u8fd9\u4e00\u6280\u672f\u7684\u6838\u5fc3\u6d41\u7a0b\u3001\u5173\u952e\u8bbe\u5907\u4e0e\u6750\u6599\uff0c\u53ca\u5176\u5728<a href=\"https:\/\/www.dxfluidics.com\/en\/microfluidic-chip\/organ-chip\/\">organ chip<\/a>\u3001\u7ec6\u80de\u6cbb\u7597\u7b49\u9886\u57df\u7684\u5e94\u7528\u524d\u666f\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"476\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72471-3.png\" alt=\"\" class=\"wp-image-5099\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72471-3.png 554w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72471-3-14x12.png 14w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"wp-block-paragraph\">\u5728\u4f20\u7edf\u65b9\u6cd5\u4e2d\uff0c\u4f7f\u7528\u4e0d\u542b\u4e19\u916e\u9178\/\u5c3f\u82f7\uff08PU\uff09\u7684\u57f9\u517b\u57fa\u8fdb\u884c\u9009\u62e9\u6027\u57f9\u517b\u5bf9\u4e8e\u751f\u6210\u8f6c\u7ebf\u7c92\u4f53\u80de\u8d28\u6742\u79cd\u81f3\u5173\u91cd\u8981\u3002\u5728\u6b64\uff0c\u6211\u4eec\u4ecb\u7ecd\u4e00\u79cd\u5229\u7528\u5fae\u6d41\u63a7\u88c5\u7f6e\u751f\u6210\u8f6c\u7ebf\u7c92\u4f53\u80de\u8d28\u6742\u79cd\u7684\u65b9\u6848\uff0c\u8be5\u65b9\u6848\u5373\u4f7f\u5728\u6dfb\u52a0PU\u7684\u6761\u4ef6\u4e0b\u4e5f\u80fd\u53d1\u6325\u4f5c\u7528\u3002\u6211\u4eec\u63cf\u8ff0\u4e86\u5fae\u6d41\u63a7\u88c5\u7f6e\u7684\u5236\u5907\u3001\u90e8\u5206\u7ec6\u80de\u878d\u5408\uff08\u7ebf\u7c92\u4f53\u8f6c\u79fb\uff09\u4ee5\u53ca\u8f6c\u7ebf\u7c92\u4f53\u80de\u8d28\u6742\u79cd\u7684\u6536\u96c6\u6b65\u9aa4\u3002\u968f\u540e\uff0c\u6211\u4eec\u8be6\u7ec6\u8bf4\u660e\u4e86\u901a\u8fc7\u9650\u5236\u6027\u7247\u6bb5\u957f\u5ea6\u591a\u6001\u6027\uff08RFLP\uff09\u5206\u6790\u786e\u8ba4\u80de\u8d28\u6742\u79cd\u4e2d\u7ebf\u7c92\u4f53DNA\uff08mtDNA\uff09\u91cd\u65b0\u805a\u96c6\u548c\u8fdb\u884cmtDNA\u5206\u578b\u7684\u6d41\u7a0b\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">1.&nbsp;&nbsp;&nbsp; \u7ebf\u7c92\u4f53\u6742\u5408\u7ec6\u80de\u5236\u5907\u7684\u6280\u672f\u7a81\u7834\uff1a\u4ece\u4f20\u7edf\u65b9\u6cd5\u5230\u5fae\u6d41\u63a7\u9769\u65b0<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u7ebf\u7c92\u4f53\u6742\u5408\u7ec6\u80de\u4f5c\u4e3a\u7814\u7a76\u7ebf\u7c92\u4f53\u529f\u80fd\u4e0e\u75be\u75c5\u5173\u8054\u7684\u91cd\u8981\u6a21\u578b\uff0c\u5176\u4f20\u7edf\u5236\u5907\u9700\u901a\u8fc7\u53bb\u6838\u4f9b\u4f53\u7ec6\u80de\u4e0e mtDNA \u7f3a\u9677\u53d7\u4f53\u7ec6\u80de\u878d\u5408\uff0c\u4f9d\u8d56\u65e0 PU \u57f9\u517b\u57fa\u7b5b\u9009 \u2014\u2014 \u56e0 mtDNA \u7f3a\u9677\u7ec6\u80de\uff08\u03c1\u2070\u7ec6\u80de\uff09\u65e0\u6cd5\u81ea\u4e3b\u5408\u6210\u4e19\u916e\u9178\u548c\u5c3f\u82f7\uff0c\u4ec5\u878d\u5408\u7ec6\u80de\u53ef\u5b58\u6d3b\u3002\u4f46\u8be5\u65b9\u6cd5\u5b58\u5728\u7b5b\u9009\u5468\u671f\u957f\u3001\u7ec6\u80de\u6d3b\u6027\u4f4e\u7b49\u95ee\u9898\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5fae\u6d41\u63a7\u6280\u672f\u7684\u4ecb\u5165\u5b9e\u73b0\u4e86\u4e09\u5927\u7a81\u7834\uff1a<strong>\u4e00\u662f<\/strong><strong> PU <\/strong><strong>\u8865\u5145\u6761\u4ef6\u4e0b\u7684\u9009\u62e9\u6027\u57f9\u517b<\/strong>\uff0c\u901a\u8fc7\u5fae\u901a\u9053\u7cbe\u51c6\u63a7\u5236\u7ec6\u80de\u5fae\u73af\u5883\uff0c\u65e0\u9700\u4f9d\u8d56\u8425\u517b\u7f3a\u9677\u7b5b\u9009\uff1b<strong>\u4e8c\u662f\u5355\u7ec6\u90e8\u5206\u878d\u5408<\/strong>\uff0c\u5229\u7528\u5fae\u6d41\u63a7\u82af\u7247\u7684\u7ec6\u80de\u914d\u5bf9\u7ed3\u6784\uff08CPS\uff09\u4e0e\u5fae\u96a7\u9053\u8bbe\u8ba1\uff0c\u5b9e\u73b0\u7ebf\u7c92\u4f53\u5b9a\u5411\u8f6c\u79fb\u800c\u907f\u514d\u7ec6\u80de\u6838\u878d\u5408\uff1b<strong>\u4e09\u662f\u9ad8\u6548\u7b5b\u9009\u4e0e\u9274\u5b9a<\/strong>\uff0c\u7ed3\u5408 PCR \u4e0e\u9650\u5236\u6027\u7247\u6bb5\u957f\u5ea6\u591a\u6001\u6027\uff08RFLP\uff09\u5206\u6790\uff0c\u5feb\u901f\u786e\u8ba4 mtDNA \u6765\u6e90\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u8fd9\u4e00\u6280\u672f\u4f9d\u8d56<strong><a href=\"https:\/\/www.dxfluidics.com\/en\/microfluidic-device\/pdms\u82af\u7247\u52a0\u5de5\u8bbe\u5907\/\">PDMS \u82af\u7247\u52a0\u5de5<\/a><\/strong>\u7684\u9ad8\u7cbe\u5ea6\u5fae\u7ed3\u6784 \u2014\u2014 \u901a\u8fc7\u8f6f\u5149\u523b\u6280\u672f\u5236\u5907\u7684\u5fae\u96a7\u9053\uff08\u957f\u5ea6 4-10\u03bcm\uff09\u4e0e\u7ec6\u80de\u53e3\u888b\uff0c\u4e3a\u4f9b\u4f53\uff08\u542b mtDNA \u7684 Pur\u1d63\u7ec6\u80de\uff09\u4e0e\u53d7\u4f53\uff08\u03c1\u2070\u7ec6\u80de\uff09\u63d0\u4f9b\u53ef\u63a7\u63a5\u89e6\u7a7a\u95f4\uff0c\u914d\u5408 HVJ-E \u75c5\u6bd2\u4ecb\u5bfc\u7684\u878d\u5408\u4f53\u7cfb\uff0c\u5b9e\u73b0\u9ad8\u6548\u7ebf\u7c92\u4f53\u8f6c\u79fb\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">2.&nbsp;&nbsp;&nbsp; \u6838\u5fc3\u5b9e\u9a8c\u6d41\u7a0b\uff1a\u4ece\u7ec6\u80de\u51c6\u5907\u5230\u6742\u5408\u7ec6\u80de\u6536\u83b7<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">1)&nbsp;&nbsp;&nbsp; \u4f9b\u4f53\u4e0e\u53d7\u4f53\u7ec6\u80de\u7684\u7cbe\u51c6\u5236\u5907<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u5b9e\u9a8c\u9700\u4e24\u7c7b\u7ec6\u80de\uff1a<strong>\u4e00\u662f\u7a33\u5b9a\u8868\u8fbe\u560c\u5464\u9709\u7d20\u6297\u6027\u57fa\u56e0\uff08<\/strong><strong>Pur<\/strong><strong>\u1d63<\/strong><strong>\uff09\u7684\u4f9b\u4f53\u7ec6\u80de<\/strong>\uff08\u5982 HeLa \u7ec6\u80de\uff09\uff0c\u4e8c\u662f mtDNA \u5b8c\u5168\u7f3a\u5931\u7684 \u03c1\u2070\u53d7\u4f53\u7ec6\u80de\uff08\u5982 143B \u7ec6\u80de\uff09\u3002\u03c1\u2070\u7ec6\u80de\u5236\u5907\u9700\u7ecf\u6eb4\u5316\u4e59\u952d\uff08EtBr\uff09\u5904\u7406 30-60 \u5929\uff0c\u6bcf\u65e5\u66f4\u6362\u57f9\u517b\u57fa\u4ee5\u6e05\u9664 mtDNA\uff0c\u540e\u7eed\u5728 PU \u57f9\u517b\u57fa\u4e2d\u57f9\u517b 1 \u4e2a\u6708\u4ee5\u4e0a\uff0c\u901a\u8fc7 PCR \u786e\u8ba4\u65e0 mtDNA \u6b8b\u7559\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5173\u952e\u64cd\u4f5c\u5305\u62ec\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u4f9b\u4f53\u7ec6\u80de\u9700\u8f6c\u5165\u8367\u5149\u6807\u8bb0\u57fa\u56e0\uff08\u5982 H2B-EGFP\uff09\uff0c\u53d7\u4f53\u7ec6\u80de\u8f6c\u5165 mCherry \u6807\u8bb0\uff0c\u4fbf\u4e8e\u540e\u7eed\u8ffd\u8e2a\uff1b<\/li>\n\n\n\n<li>\u786e\u4fdd\u4f9b\u4f53\u4e3a TK\u207a\u3001\u53d7\u4f53\u4e3a TK\u207b\uff0c\u901a\u8fc7 BrdU \u7b5b\u9009\u6e05\u9664\u672a\u878d\u5408\u7684\u4f9b\u4f53\u7ec6\u80de\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">2)&nbsp;&nbsp;&nbsp; \u5fae\u6d41\u63a7\u82af\u7247\u7684\u5236\u5907\u5de5\u827a<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u82af\u7247\u5236\u5907\u4ee5<strong><a href=\"https:\/\/www.dxfluidics.com\/en\/product\/consumables\/895\/pdms-pdms\u8584\u819c-pdms\u5fae\u5b54\u819c\/\">PDMS \u6750\u6599<\/a><\/strong>\u4e3a\u6838\u5fc3\uff0c\u901a\u8fc7\u8f6f\u5149\u523b\u6280\u672f\u5b9e\u73b0\u5fae\u7ed3\u6784\u590d\u523b\uff0c\u5177\u4f53\u6d41\u7a0b\u5305\u62ec\uff1a<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"565\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72472-3.png\" alt=\"\" class=\"wp-image-5100\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72472-3.png 554w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72472-3-12x12.png 12w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"has-text-align-center wp-block-paragraph\">\u4e3b\u6a21\u5177\u5236\u9020\u6d41\u7a0b\u793a\u610f\u56fe<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u5149\u523b\u80f6\u6a21\u5177\u5236\u4f5c<\/strong>\uff1a\u5728\u7845\u7247\u4e0a\u6d82\u8986 <a href=\"https:\/\/www.dxfluidics.com\/en\/product\/consumables\/906\/su-8\u5149\u523b\u80f6-\u663e\u5f71\u6db2\/\">SU-8 \u5149\u523b\u80f6<\/a>\uff0c\u7ecf\u7d2b\u5916\u66dd\u5149\u3001\u663e\u5f71\u5f62\u6210\u5fae\u96a7\u9053\uff08\u7b2c\u4e00\u5c42\uff0c2\u03bcm \u539a\uff09\u4e0e\u7ec6\u80de\u914d\u5bf9\u7ed3\u6784\uff08\u7b2c\u4e8c\u5c42\uff0c26-28\u03bcm \u539a\uff09\uff0c\u9700\u7cbe\u786e\u5bf9\u51c6\u4e24\u5c42\u56fe\u6848\uff08\u8bef\u5dee &lt; 0.5\u03bcm\uff09\uff1b<\/li>\n\n\n\n<li><strong>PDMS <\/strong><strong>\u6d47\u7b51\u4e0e\u56fa\u5316<\/strong>\uff1a\u6309 10:1 \u6bd4\u4f8b\u6df7\u5408 PDMS \u9884\u805a\u4f53\u4e0e\u4ea4\u8054\u5242\uff0c\u5012\u5165\u6a21\u5177\u771f\u7a7a\u8131\u6ce1\u540e\uff0c65\u2103\u70d8\u70e4 1 \u5c0f\u65f6\u56fa\u5316\uff0c\u5207\u5272\u540e\u6253\u5b54\u5f62\u6210\u8fdb\u51fa\u6db2\u53e3\uff1b<\/li>\n\n\n\n<li><strong>\u82af\u7247\u7ec4\u88c5<\/strong>\uff1a\u5c06 <a href=\"https:\/\/www.dxfluidics.com\/en\/product\/microfluidic-chip\/custom-chip\/146\/pdms\u82af\u7247\/\">PDMS \u82af\u7247<\/a>\u4e0e\u73bb\u7483\u8f7d\u7247\u53ef\u9006\u7ed3\u5408\uff0c\u907f\u514d\u7b49\u79bb\u5b50\u952e\u5408\u5bfc\u81f4\u7684\u7ec6\u80de\u7c98\u9644\u5e72\u6270\uff0c\u786e\u4fdd\u5fae\u901a\u9053\u5bc6\u5c01\u6027\u3002<\/li>\n<\/ul>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"410\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72473-3.png\" alt=\"\" class=\"wp-image-5101\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72473-3.png 554w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72473-3-16x12.png 16w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"has-text-align-center wp-block-paragraph\">\u5149\u63a9\u6a21\u8bbe\u8ba1\u603b\u7ed3<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\uff08A\uff09\u7ec6\u80de\u914d\u5bf9\u7ed3\u6784\uff08CPS\uff09\u7684\u53d8\u5316\u3002\u5fae\u96a7\u9053\u957f\u5ea6\u7531\u7bad\u5934\u6240\u793a\u7684\u7ec6\u80de\u51f9\u7a74\u4e4b\u95f4\u7684\u8ddd\u79bb\u5b9a\u4e49\u3002\u6bd4\u4f8b\u5c3a\uff1a30\u5fae\u7c73\u3002\uff08B\uff09\u7845\u7247\u3001\u7b2c\u4e00\u5c42\uff08\u5fae\u96a7\u9053\uff09\u548c\u7b2c\u4e8c\u5c42\uff08CPS\uff09\u4e4b\u95f4\u7684\u5bf9\u51c6\u6982\u8ff0\u3002\u900f\u8fc7\u5bf9\u51c6\u6807\u8bb0\u7a97\u53e3\u53ef\u4ee5\u770b\u5230\u7845\u7247\u4e0a\u7684\u5bf9\u51c6\u6807\u8bb0\u3002\u7bad\u5934\u56fe\u6848\u6709\u52a9\u4e8e\u786e\u5b9a\u5bf9\u51c6\u7684Y\u8f74\u4f4d\u7f6e\u3002\uff08C\uff09CPS\/\u5fae\u96a7\u9053\u8bbe\u8ba1\u7684\u653e\u5927\u56fe\u3002\u663e\u793a\u4e86\u5e26\u6709\u77ed\uff084\u5fae\u7c73\u957f\uff09\u5fae\u96a7\u9053\u7684CPS\u3002\u5173\u4e8e\u5149\u63a9\u6a21\u8bbe\u8ba1\u7684\u8be6\u7ec6\u4fe1\u606f\uff0c\u8bf7\u53c2\u89c1\u56fe1\u3001\u56fe2\u3001\u56fe3\u4ee5\u53ca\u6240\u63d0\u4f9b\u7684CAD\u6570\u636e\uff08dwg\u6587\u4ef6\uff1a\u63a9\u6a21#4\u3001#20\u548c#27\uff09\u3002\u6bd4\u4f8b\u5c3a\uff1a30\u5fae\u7c73\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"444\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72474-3.png\" alt=\"\" class=\"wp-image-5102\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72474-3.png 554w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72474-3-15x12.png 15w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"has-text-align-center wp-block-paragraph\">\u5fae\u6d41\u63a7\u88c5\u7f6e\u5236\u5907\u6d41\u7a0b\u793a\u610f\u56fe<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\uff08A\uff09\u7a7a\u7684\u4e3b\u6a21\u5177\u3002\uff08B-D\uff09PDMS\u82af\u7247\u5236\u5907\u6b65\u9aa4\uff08\u6b65\u9aa42-7\uff09\u3002\uff08E\uff09\u6536\u96c6\u7684PDMS\u82af\u7247\u3002\uff08F-I\uff09\u5fae\u6d41\u63a7\u88c5\u7f6e\u5236\u5907\u6b65\u9aa4\uff08\u6b65\u9aa410-14\uff09\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"489\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72475-2.png\" alt=\"\" class=\"wp-image-5103\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72475-2.png 554w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72475-2-14x12.png 14w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"wp-block-paragraph\">\uff08A\uff09\u5207\u5272\u56fa\u5316\u7684PDMS\uff08\u6b65\u9aa46\uff09\u3002\uff08B\uff09\u5265\u79bbPDMS\uff08\u6b65\u9aa47\uff09\u3002\uff08C\uff09\u6e05\u7a7a\u6bcd\u6a21\u3002\u5efa\u8bae\u5c06\u6bcd\u6a21\u7a7a\u7f6e\u5b58\u653e\uff0c\u4ee5\u907f\u514dPDMS\u7c98\u5728\u7845\u7247\u4e0a\u3002\uff08D\uff09\u5c06PDMS\u82af\u7247\u653e\u7f6e\u5728\u5e72\u51c0\u7684\u8f7d\u73bb\u7247\u4e0a\uff08\u6b65\u9aa48\uff09\u3002\uff08E\u2013G\uff09\u4fee\u526a\u5e76\u6253\u5b54\u4ee5\u5236\u4f5c\u51fa\u53e3\u548c\u5165\u53e3\uff08\u6b65\u9aa410\uff09\u3002\uff08H\uff09\u8fde\u63a5\u7845\u80f6\u7ba1\u4ee5\u5236\u4f5c\u5165\u53e3\u50a8\u6db2\u5668\uff08\u6b65\u9aa411\u201313\uff09\u3002\uff08I\uff09\u5c06PDMS\u82af\u7247\u653e\u7f6e\u572835\u6beb\u7c73\u57f9\u517b\u76bf\u4e2d\uff08\u6b65\u9aa414\uff09\u3002\uff08J\uff09\u7531\u4e8e\u65e0\u9700\u8fde\u63a5\u4efb\u4f55\u5916\u90e8\u8bbe\u5907\uff0c\u5fae\u6d41\u63a7\u88c5\u7f6e\u53ef\u914d\u5907\u76d6\u5b50\u3002G-I\u4e2d\u6240\u793a\u7684PDMS\u82af\u7247\u5e76\u4e0d\u5b8c\u5168\u76f8\u540c\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">3)&nbsp;&nbsp;&nbsp; \u90e8\u5206\u7ec6\u80de\u878d\u5408\u4e0e\u6742\u5408\u7ec6\u80de\u7b5b\u9009<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">\u878d\u5408\u8fc7\u7a0b\u901a\u8fc7<strong>\u5fae\u6d41\u63a7\u82af\u7247\u7684\u6d41\u4f53\u63a7\u5236<\/strong>\u5b9e\u73b0\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u7ec6\u80de\u60ac\u6db2\uff080.5-1\u00d710\u2075 cells\/100\u03bcL\uff09\u6ce8\u5165\u82af\u7247\uff0c440\u00d7g \u79bb\u5fc3 1 \u5206\u949f\u4f7f\u7ec6\u80de\u8fdb\u5165 CPS\uff0c\u5f62\u6210 1:1 \u914d\u5bf9\uff1b<\/li>\n\n\n\n<li>\u6ce8\u5165\u542b HVJ-E \u4e0e Y-27632\uff08ROCK \u6291\u5236\u5242\uff09\u7684\u878d\u5408\u57f9\u517b\u57fa\uff0c37\u2103\u57f9\u517b 1 \u5c0f\u65f6\u4fc3\u8fdb\u80de\u8d28\u6865\u5f62\u6210\uff0c\u5b9e\u73b0\u7ebf\u7c92\u4f53\u8f6c\u79fb\uff1b<\/li>\n\n\n\n<li>\u7b5b\u9009\u5206\u4e24\u6b65\uff1a\u5148\u7528 Pur \u57f9\u517b\u57fa\u6e05\u9664\u672a\u878d\u5408\u53d7\u4f53\u7ec6\u80de\uff0c\u518d\u7528 PU\/BrdU \u57f9\u517b\u57fa\u6dd8\u6c70\u4f9b\u4f53\u7ec6\u80de\uff0c\u6700\u7ec8\u83b7\u5f97\u7eaf\u5408\u6742\u5408\u7ec6\u80de\u514b\u9686\u3002<\/li>\n<\/ul>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"450\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72476-2.png\" alt=\"\" class=\"wp-image-5104\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72476-2.png 554w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72476-2-15x12.png 15w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"has-text-align-center wp-block-paragraph\">\u901a\u8fc7\u5fae\u96a7\u9053\u8fdb\u884c\u7ec6\u80de\u878d\u5408\u7684\u6d41\u7a0b\u793a\u610f\u56fe \u5728\u8be5\u793a\u610f\u56fe\u4e2d\uff0c\u7ebf\u7c92\u4f53\u672a\u88ab\u663e\u793a\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">3.&nbsp;&nbsp;&nbsp; \u5173\u952e\u6280\u672f\u4e0e\u8bbe\u5907\uff1a\u4ece\u5fae\u7eb3\u52a0\u5de5\u5230\u68c0\u6d4b\u7cfb\u7edf<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">1)&nbsp;&nbsp;&nbsp; \u5fae\u6d41\u63a7\u82af\u7247\u52a0\u5de5\u8bbe\u5907<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong><a href=\"https:\/\/www.dxfluidics.com\/en\/product\/microfluidic-device\/desktop-lithography-machine-exposure-machine\/dingxu-scientific-research-exposure-machine\/1270\/\u684c\u9762\u5149\u523b\u673a-\u66dd\u5149\u673a\/\">\u5149\u523b\u7cfb\u7edf<\/a><\/strong>\uff1a\u7d2b\u5916\u5149\u523b\u673a\uff08\u5982 Union Optical PEM-800\uff09\u7528\u4e8e SU-8 \u56fe\u6848\u66dd\u5149\uff0c\u9700\u914d\u5408\u9540\u94ec\u73bb\u7483\u63a9\u819c\uff08\u7cbe\u5ea6\u8fbe \u03bcm \u7ea7\uff09\uff1b<\/li>\n\n\n\n<li><strong>\u7b49\u79bb\u5b50\u523b\u8680\u673a<\/strong>\uff1a\u7528\u4e8e\u7845\u7247\u8868\u9762\u5904\u7406\u4e0e\u5fae\u7ed3\u6784\u4fee\u9970\uff0c\u901a\u8fc7 O\u2082\u4e0e CHF\u2083\u7b49\u79bb\u5b50\u4f53\u5b9e\u73b0\u4eb2\u758f\u6c34\u8c03\u63a7\uff1b<\/li>\n\n\n\n<li><strong><a href=\"https:\/\/www.dxfluidics.com\/en\/microfluidic-device\/pdms\u82af\u7247\u52a0\u5de5\u8bbe\u5907\/\">PDMS \u52a0\u5de5\u8bbe\u5907<\/a><\/strong>\uff1a\u5305\u62ec\u5300\u80f6\u673a\uff08\u63a7\u5236\u5149\u523b\u80f6\u539a\u5ea6\uff09\u3001\u70d8\u7bb1\uff0865\u2103\u56fa\u5316\uff09\u3001\u6253\u5b54\u5668\uff082mm \u76f4\u5f84 biopsy trepan\uff09\u7b49\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">2)&nbsp;&nbsp;&nbsp; \u7ec6\u80de\u64cd\u4f5c\u4e0e\u68c0\u6d4b\u5de5\u5177<\/h3>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"410\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72477-1.png\" alt=\"\" class=\"wp-image-5105\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72477-1.png 554w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72477-1-16x12.png 16w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"has-text-align-center wp-block-paragraph\">\u90e8\u5206\u7ec6\u80de\u878d\u5408\u7684\u5b9e\u73b0\u53ca\u540e\u7eed\u5206\u79bb<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5728\u672c\u5b9e\u9a8c\u4e2d\uff0c\u4f7f\u7528\u4e86\u5177\u6709105\u4e2aCPS\u4e14\u5e26\u6709\u77ed\uff084\u03bcm\u957f\uff09\u5fae\u901a\u9053\u7684\u5fae\u6d41\u63a7\u88c5\u7f6e\u3002\uff08A\uff09\u5b9e\u9a8c\u6982\u8ff0\u3002\u5728CPS\u4e2d\u6355\u83b7\u8868\u8fbeH2B-EGFP\u7684HeLa\u7ec6\u80de\uff08\u4f9b\u4f53\u7ec6\u80de\uff09\u548c\u8868\u8fbemCherry\u7684\\(143B\\)\u7ec6\u80de\uff08\u53d7\u4f53\u7ec6\u80de\uff09\u540e\uff0c\u901a\u5165\u878d\u5408\u57f9\u517b\u57fa1\u5c0f\u65f6\uff0c\u7136\u540e\u901a\u5165Pur\u9009\u62e9\u57f9\u517b\u57fa1\u5929\u3002\u672c\u793a\u610f\u56fe\u4e2d\u7701\u7565\u4e86\u7ebf\u7c92\u4f53\u3002\uff08B\uff09\u878d\u5408\u7ec6\u80de\u5bf9\u7684\u8367\u5149\u56fe\u50cf\u3002\u5728\u672c\u5b9e\u9a8c\u4e2d\uff0c\u7ebf\u7c92\u4f53DNA\u672a\u88ab\u53ef\u89c6\u5316\u3002\u7ec6\u80de\u8d28\u4e2d\u7684mCherry\u4ece\\(\\rho^{0} 143 B\\)\u7ec6\u80de\u8f6c\u79fb\u5230HeLa\u7ec6\u80de\uff0c\u8868\u660e\u5b83\u4eec\u4e4b\u95f4\u53d1\u751f\u4e86\u90e8\u5206\u7ec6\u80de\u878d\u5408\u3002H2B-EGFP\u4fe1\u53f7\u548cmCherry\u4fe1\u53f7\u5206\u522b\u4ee5\u7eff\u8272\u548c\u7ea2\u8272\u663e\u793a\u3002\u76f8\u5dee\uff1a\u76f8\u5dee\u663e\u5fae\u955c\u56fe\u50cf\u3002\u878d\u5408\u524d\uff1a\u7ec6\u80de\u6355\u83b7\u540e\u7acb\u5373\u62cd\u6444\u3002\u878d\u5408\u540e\uff1a\u901a\u5165\u878d\u5408\u57f9\u517b\u57fa1\u5c0f\u65f6\u540e\u7acb\u5373\u62cd\u6444\u3002\u6240\u6709\u56fe\u50cf\u653e\u5927\u500d\u6570\u76f8\u540c\u3002\u6bd4\u4f8b\u5c3a\uff1a25\u03bcm\u3002\uff08C\uff09B\u4e2d\u6240\u793a\u878d\u5408\u7ec6\u80de\u5bf9\u7684\u5ef6\u65f6\u89c2\u5bdf\u7ed3\u679c\u3002\u5b9e\u7ebf\u7bad\u5934\u548c\u865a\u7ebf\u7bad\u5934\u5206\u522b\u8868\u793aHeLa\u7ec6\u80de\u548c\u03c10143B\u7ec6\u80de\u3002\u661f\u53f7\u8868\u793a\u4eceCPS\u5916\u90e8\u79fb\u5165\u7684\u7ec6\u80de\u3002\u5728\u901a\u5165Pur\u9009\u62e9\u57f9\u517b\u57fa\u7684\u8fc7\u7a0b\u4e2d\uff0c\u878d\u5408\u7ec6\u80de\u81ea\u53d1\u5206\u79bb\uff0c\u6062\u590d\u4e3a\u5355\u4e2a\u7ec6\u80de\u3002\u6240\u6709\u56fe\u50cf\u653e\u5927\u500d\u6570\u76f8\u540c\u3002\u6bd4\u4f8b\u5c3a\uff1a50\u03bcm\u3002\u90e8\u5206\u6570\u636e\u5df2\u5728Wada\u7b49\u4eba\u7684\u7814\u7a76\u4e2d\u53d1\u8868\uff0c1 \u6b64\u5904\u6839\u636e\u51fa\u7248\u5546\u7684\u7248\u6743\u653f\u7b56\u8fdb\u884c\u91cd\u73b0\u3002\u672c\u6587\u5df2\u83b7\u5f97CC BY\u8bb8\u53ef\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"393\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72478-1.png\" alt=\"\" class=\"wp-image-5106\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72478-1.png 554w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72478-1-18x12.png 18w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"wp-block-paragraph\">\u4f7f\u7528\u4e86\u5177\u6709105\u4e2aCPS\u3001\u5e26\u6709\u77ed\uff084\u03bcm\u957f\uff09\u5fae\u96a7\u9053\u6216\u65e0\u5fae\u96a7\u9053\uff08\u65e0\u96a7\u9053\uff09\u7684\u88c5\u7f6e\u3002\uff08\u53d7\u4f53\uff09\u7ec6\u80de\u3002\u5b9e\u9a8c\u4f7f\u7528\u8868\u8fbeH2B-EGFP\u7684HeLa\u7ec6\u80de\uff08\u4f9b\u4f53\uff09\u548c\u8868\u8fbeH2B-mCherry\u7684\\(\\rho^{0} 143 B\\)\u7ec6\u80de\u8fdb\u884c\u3002\uff08B\uff09\u7b2c19\u5929\u7528\u65b9\u6cd52\u7b5b\u9009\u7684\u5178\u578b\u7ed3\u679c\uff08\u53e6\u89c1\u56fe7\uff09\u3002\u5728\u8fd9\u4e9b\u5b9e\u9a8c\u4e2d\uff0c\u5fae\u6d41\u63a7\uff08A\uff09\u7b2c8\u5929\u7528\u65b9\u6cd51\u7b5b\u9009\u7684\u5178\u578b\u7ed3\u679c\uff08\u53e6\u89c1\u56fe7\uff09\u3002\u672c\u5b9e\u9a8c\u4f7f\u7528\u5177\u67091260\u4e2aCPS\u3001\u5e26\u6709\u957f\uff0810\u03bcm\u957f\uff09\u96a7\u9053\u7684\u5fae\u6d41\u63a7\u88c5\u7f6e\u3002\u5de6\u56fe\uff1a\u7ec6\u80de\u7684\u76f8\u5dee\u663e\u5fae\u955c\u56fe\u50cf\u3002\u7bad\u5934\u6307\u793a\u51fa\u73b0\u7684\u96c6\u843d\u3002\u6b64\u65f6\uff0c\u4f9b\u4f53\u7ec6\u80de\u4ecd\u7136\u5b58\u6d3b\u3002\u968f\u540e\u901a\u8fc7\u4f20\u4ee3\u7b5b\u9009\uff0c\u4f9b\u4f53\u7ec6\u80de\u88ab\u5b8c\u5168\u6e05\u9664\uff08\u6570\u636e\u672a\u663e\u793a\uff09\u3002\u6bd4\u4f8b\u5c3a\uff1a500\u03bcm\u3002\u53f3\u56fe\uff1a\u96c6\u843d\u7684\u653e\u5927\u76f8\u5dee\u663e\u5fae\u955c\u56fe\u50cf\u548c\u8367\u5149\u56fe\u50cf\u3002\u56fe11. \u9009\u62e9\u6027\u57f9\u517b\u671f\u95f4\u51fa\u73b0\u7684\u96c6\u843d\u56fe\u50cf\u4ee5\u76f8\u540c\u653e\u5927\u500d\u6570\u663e\u793a\u3002\u6bd4\u4f8b\u5c3a\uff1a500\u03bcm\u3002\u5de6\u56fe\uff1a\u4f7f\u7528\u77ed\u96a7\u9053\u88c5\u7f6e\u7684\u5b9e\u9a8c\u4e2d\u7ec6\u80de\u7684\u76f8\u5dee\u663e\u5fae\u955c\u56fe\u50cf\u3002\u53f3\u56fe\uff1a\u4f7f\u7528\u65e0\u96a7\u9053\u88c5\u7f6e\u7684\u5b9e\u9a8c\u4e2d\u7ec6\u80de\u7684\u76f8\u5dee\u663e\u5fae\u955c\u56fe\u50cf\u3002\u77ed\u96a7\u9053\u88c5\u7f6e\u4e2d\u51fa\u73b0\u660e\u663e\u7684\u96c6\u843d\uff0c\u800c\u65e0\u96a7\u9053\u88c5\u7f6e\u4e2d\u672a\u51fa\u73b0\u3002\u7eff\u8272\uff1a\u4f9b\u4f53\u7ec6\u80de\u7684\u7ec6\u80de\u6838\uff08H2B-EGFP\uff09\uff0c\u7ea2\u8272\uff1a\u53d7\u4f53\u7ec6\u80de\u7684\u7ec6\u80de\u6838\uff08H2B-mCherry\uff09\u3002\u56fe\u50cf\u4ee5\u76f8\u540c\u653e\u5927\u500d\u6570\u663e\u793a\u3002\u6bd4\u4f8b\u5c3a\uff1a100\u03bcm\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"554\" height=\"419\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72479-1.png\" alt=\"\" class=\"wp-image-5107\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72479-1.png 554w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2025\/09\/\u56fe\u72479-1-16x12.png 16w\" sizes=\"auto, (max-width: 554px) 100vw, 554px\" \/><\/figure>\n<\/div>\n\n\n<p class=\"wp-block-paragraph\">\u901a\u8fc7\u8367\u5149\u663e\u5fae\u955c\u89c2\u5bdf\u786e\u8ba4\u7ebf\u7c92\u4f53DNA\u7684\u5b58\u5728 \u8868\u8fbeH2B-EGFP\u7684HeLa\u7ec6\u80de\uff08\u4f9b\u4f53\uff09\u3001\u8868\u8fbeH2B-mCherry\u7684\\(p143B\\)\u7ec6\u80de\uff08\u53d7\u4f53\uff09\u53ca\u6240\u5f97\u80de\u8d28\u6742\u79cd\u7684\u8367\u5149\u56fe\u50cf\u3002\u6240\u6709\u7ec6\u80de\u5747\u7528PicoGreen\u67d3\u8272\u3002\u672c\u5b9e\u9a8c\u4e2d\uff0c\u4f7f\u7528\u77ed\uff084\u03bcm\u957f\uff09\u901a\u9053\u88c5\u7f6e\u901a\u8fc7\u65b9\u6cd52\u751f\u6210\u80de\u8d28\u6742\u79cd\uff08\u53e6\u89c1\u56fe7\uff09\u3002\u80de\u8d28\u6742\u79cd\u4e2d\u5b58\u5728\u7ec6\u80de\u8d28PicoGreen\u4fe1\u53f7\u8868\u660e\u7ebf\u7c92\u4f53DNA\u91cd\u65b0\u805a\u96c6\u3002PG\uff1aPicoGreen\u3002\u56fe\u50cf\u4ee5\u76f8\u540c\u653e\u5927\u500d\u6570\u663e\u793a\u3002\u6807\u5c3a\uff1a50\u03bcm\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u8367\u5149\u663e\u5fae\u955c<\/strong>\uff1a\u5982 Olympus IX81\uff0c\u7528\u4e8e\u89c2\u5bdf EGFP\/mCherry \u6807\u8bb0\u7684\u7ec6\u80de\u878d\u5408\u72b6\u6001\uff1b<\/li>\n\n\n\n<li><strong>PCR <\/strong><strong>\u4e0e\u7535\u6cf3\u7cfb\u7edf<\/strong>\uff1a\u901a\u8fc7 KOD \u9176\u6269\u589e mtDNA \u7247\u6bb5\uff0c\u7ed3\u5408 SspI \u9176\u5207\u4e0e\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\uff0c\u533a\u5206 HeLa\uff081854bp\uff09\u4e0e 143B\uff081289bp\uff09\u6765\u6e90\u7684 mtDNA\uff1b<\/li>\n\n\n\n<li><strong>\u7ec6\u80de\u57f9\u517b\u8bbe\u5907<\/strong>\uff1aCO\u2082\u57f9\u517b\u7bb1\u9700\u7ef4\u6301 5% CO\u2082\u4e0e 37\u2103\u73af\u5883\uff0c\u914d\u5408 PU \u57f9\u517b\u57fa\uff08\u542b 1mM \u4e19\u916e\u9178\u30011\u03bcg\/mL \u5c3f\u82f7\uff09\u5b9e\u73b0\u6742\u5408\u7ec6\u80de\u6269\u589e\u3002<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">4.&nbsp;&nbsp;&nbsp; \u5e94\u7528\u524d\u666f\u4e0e\u6280\u672f\u6269\u5c55<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>1<\/strong><strong>\uff09. <\/strong><strong>\u75be\u75c5\u6a21\u578b\u4e0e\u836f\u7269\u7814\u53d1<\/strong><\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">\u8be5\u6280\u672f\u53ef\u6784\u5efa\u542b\u7279\u5b9a mtDNA \u7a81\u53d8\u7684\u6742\u5408\u7ec6\u80de\uff0c\u6a21\u62df\u7ebf\u7c92\u4f53\u75be\u75c5\uff08\u5982 Leber \u9057\u4f20\u6027\u89c6\u795e\u7ecf\u75c5\uff09\uff0c\u7528\u4e8e\u836f\u7269\u7b5b\u9009\u3002\u7ed3\u5408<strong>organoid chip<\/strong>\uff0c\u53ef\u8fdb\u4e00\u6b65\u6784\u5efa \u201c\u6742\u5408\u7ec6\u80de &#8211; \u7c7b\u5668\u5b98\u201d \u6a21\u578b\uff0c\u7814\u7a76\u7ebf\u7c92\u4f53\u529f\u80fd\u5bf9\u7ec4\u7ec7\u53d1\u80b2\u7684\u5f71\u54cd\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>2<\/strong><strong>\uff09. <\/strong><strong>\u5fae\u6d41\u63a7\u6280\u672f\u7684\u6a2a\u5411\u6269\u5c55<\/strong><\/li>\n\n\n\n<li><strong>\u5668\u5b98\u82af\u7247\u96c6\u6210<\/strong>\uff1a\u5c06\u7ebf\u7c92\u4f53\u8f6c\u79fb\u6a21\u5757\u4e0e\u591a\u5668\u5b98\u82af\u7247\u7ed3\u5408\uff0c\u7814\u7a76\u8de8\u5668\u5b98\u7ebf\u7c92\u4f53\u4ee3\u8c22\u534f\u540c\uff1b<\/li>\n\n\n\n<li><strong>\u81ea\u52a8\u5316\u7b5b\u9009<\/strong>\uff1a\u7ed3\u5408<strong>\u6570\u5b57\u5fae\u6d41\u63a7\u82af\u7247<\/strong>\uff0c\u5b9e\u73b0\u7ec6\u80de\u914d\u5bf9\u3001\u878d\u5408\u3001\u7b5b\u9009\u7684\u5168\u6d41\u7a0b\u81ea\u52a8\u5316\uff1b<\/li>\n\n\n\n<li><strong>3D <\/strong><strong>cell culture<\/strong>\uff1a\u5728\u82af\u7247\u4e2d\u5f15\u5165\u6c34\u51dd\u80f6\u652f\u67b6\uff0c\u6a21\u62df\u4f53\u5185\u5fae\u73af\u5883\u4e0b\u7684\u7ebf\u7c92\u4f53\u8f6c\u79fb\u6548\u7387\u3002<\/li>\n\n\n\n<li><strong>3<\/strong><strong>\uff09. <\/strong><strong>\u6280\u672f\u4f18\u5316\u65b9\u5411<\/strong><\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">\u5f53\u524d\u6311\u6218\u5305\u62ec\uff1a\u975e\u8d34\u58c1\u7ec6\u80de\u96be\u4ee5\u9002\u914d\uff08\u4f9d\u8d56\u5fae\u96a7\u9053\u7a7f\u900f\u7684\u80de\u8d28\u6865\uff09\u3001HVJ-E \u8bd5\u5242\u505c\u4ea7\uff08\u9700\u66ff\u4ee3\u75c5\u6bd2\u8f7d\u4f53\uff09\u3002\u672a\u6765\u53ef\u901a\u8fc7<strong>\u5fae\u6d41\u63a7\u82af\u7247\u8868\u9762\u4fee\u9970<\/strong>\uff08\u5982 PEG \u6d82\u5c42\uff09\u6539\u5584\u7ec6\u80de\u517c\u5bb9\u6027\uff0c\u6216\u91c7\u7528\u7535\u878d\u5408\u6280\u672f\u66ff\u4ee3\u75c5\u6bd2\u4ecb\u5bfc\u7684\u878d\u5408\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u53c2\u8003\u6587\u732e\uff1a<a href=\"https:\/\/doi.org\/10.1016\/j.\u00a0xpro.2025.103953\">https:\/\/doi.org\/10.1016\/j.\u00a0xpro.2025.103953<\/a><\/p>\n\n\n\n<h3 class=\"wp-block-heading has-pale-cyan-blue-background-color has-background has-medium-font-size\">Dxfluidics product ( standard PDMS chip)<\/h3>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1406\" height=\"943\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2024\/01\/image-26.png\" alt=\"\" class=\"wp-image-1713\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2024\/01\/image-26.png 1406w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2024\/01\/image-26-600x402.png 600w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2024\/01\/image-26-768x515.png 768w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2024\/01\/image-26-18x12.png 18w\" sizes=\"auto, (max-width: 1406px) 100vw, 1406px\" \/><\/figure>\n\n\n\n<p class=\"has-vivid-red-color has-text-color wp-block-paragraph\">\u672c\u516c\u53f8\u4e5f\u53ef\u63d0\u4f9b\u7279\u6027\u5316\u7684\u5fae\u6d41\u63a7PDMS\u82af\u7247\u52a0\u5de5\u670d\u52a1\uff0c\u5177\u4f53\u8be6\u60c5\uff0c\u6b22\u8fce\u6765\u7535\u54a8\u8be2+86-18112558497\uff08\u540c\u5fae\u4fe1\uff09\uff0c\u90ae\u7bb1:sale@dxfluidics.com<\/p>\n\n\n\n<figure class=\"wp-block-table has-small-font-size\"><table class=\"has-pale-cyan-blue-background-color has-background\"><tbody><tr><td class=\"has-text-align-center\" data-align=\"center\">Product Code<\/td><td class=\"has-text-align-center\" data-align=\"center\">Outline<br>\uff08mm\uff09<\/td><td class=\"has-text-align-center\" data-align=\"center\">Height<br>\uff08um\uff09<\/td><td class=\"has-text-align-center\" data-align=\"center\">Width<br>\uff08um\uff09<\/td><td class=\"has-text-align-center\" data-align=\"center\">Thickness<br>\uff08mm\uff09<\/td><td class=\"has-text-align-center\" data-align=\"center\">Chip material<\/td><td class=\"has-text-align-center\" data-align=\"center\">Price<br>\uff08CNY\uff09<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">K0001<\/td><td class=\"has-text-align-center\" data-align=\"center\">45*20<\/td><td class=\"has-text-align-center\" data-align=\"center\">50<\/td><td class=\"has-text-align-center\" data-align=\"center\">50&amp;100<\/td><td class=\"has-text-align-center\" data-align=\"center\">3+1<\/td><td class=\"has-text-align-center\" data-align=\"center\">PDMS+Glass<\/td><td class=\"has-text-align-center\" data-align=\"center\">300<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">K0002<\/td><td class=\"has-text-align-center\" data-align=\"center\">45*20<\/td><td class=\"has-text-align-center\" data-align=\"center\">50<\/td><td class=\"has-text-align-center\" data-align=\"center\">80&amp;100<\/td><td class=\"has-text-align-center\" data-align=\"center\">3+1<\/td><td class=\"has-text-align-center\" data-align=\"center\">PDMS+Glass<\/td><td class=\"has-text-align-center\" data-align=\"center\">300<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">K0003<\/td><td class=\"has-text-align-center\" data-align=\"center\">45*20<\/td><td class=\"has-text-align-center\" data-align=\"center\">50<\/td><td class=\"has-text-align-center\" data-align=\"center\">50&amp;100<\/td><td class=\"has-text-align-center\" data-align=\"center\">3+1<\/td><td class=\"has-text-align-center\" data-align=\"center\">PDMS+Glass<\/td><td class=\"has-text-align-center\" data-align=\"center\">300<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">K0004<\/td><td class=\"has-text-align-center\" data-align=\"center\">45*20<\/td><td class=\"has-text-align-center\" data-align=\"center\">50<\/td><td class=\"has-text-align-center\" data-align=\"center\">100&amp;300<\/td><td class=\"has-text-align-center\" data-align=\"center\">3+1<\/td><td class=\"has-text-align-center\" data-align=\"center\">PDMS+Glass<\/td><td class=\"has-text-align-center\" data-align=\"center\">300<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">K0005<\/td><td class=\"has-text-align-center\" data-align=\"center\">45*20<\/td><td class=\"has-text-align-center\" data-align=\"center\">50<\/td><td class=\"has-text-align-center\" data-align=\"center\">50&amp;100&amp;150<\/td><td class=\"has-text-align-center\" data-align=\"center\">3+1<\/td><td class=\"has-text-align-center\" data-align=\"center\">PDMS+Glass<\/td><td class=\"has-text-align-center\" data-align=\"center\">300<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">K0006<\/td><td class=\"has-text-align-center\" data-align=\"center\">45*20<\/td><td class=\"has-text-align-center\" data-align=\"center\">50<\/td><td class=\"has-text-align-center\" data-align=\"center\">100<\/td><td class=\"has-text-align-center\" data-align=\"center\">3+1<\/td><td class=\"has-text-align-center\" data-align=\"center\">PDMS+Glass<\/td><td class=\"has-text-align-center\" data-align=\"center\">300<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">K0007<\/td><td class=\"has-text-align-center\" data-align=\"center\">75*25<\/td><td class=\"has-text-align-center\" data-align=\"center\">100<\/td><td class=\"has-text-align-center\" data-align=\"center\">100&amp;300<\/td><td class=\"has-text-align-center\" data-align=\"center\">3+1<\/td><td class=\"has-text-align-center\" data-align=\"center\">PDMS+Glass<\/td><td class=\"has-text-align-center\" data-align=\"center\">300<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">K0008<\/td><td class=\"has-text-align-center\" data-align=\"center\">52*25<\/td><td class=\"has-text-align-center\" data-align=\"center\">100<\/td><td class=\"has-text-align-center\" data-align=\"center\">100&amp;150&amp;200<\/td><td class=\"has-text-align-center\" data-align=\"center\">3+1<\/td><td class=\"has-text-align-center\" data-align=\"center\">PDMS+Glass<\/td><td class=\"has-text-align-center\" data-align=\"center\">300<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">K0009<\/td><td class=\"has-text-align-center\" data-align=\"center\">35*24<\/td><td class=\"has-text-align-center\" data-align=\"center\">100<\/td><td class=\"has-text-align-center\" data-align=\"center\">100&amp;200<\/td><td class=\"has-text-align-center\" data-align=\"center\">3+1<\/td><td class=\"has-text-align-center\" data-align=\"center\">PDMS+Glass<\/td><td class=\"has-text-align-center\" data-align=\"center\">300<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\">The prices listed are for mainland China and are in Chinese Yuan (CNY,\u00a5). For regions outside mainland China, please contact customer service for a quote.(sale@dxfluidics.com)<\/figcaption><\/figure>\n\n\n\n<h3 class=\"wp-block-heading has-pale-cyan-blue-background-color has-background has-medium-font-size\">Ordering Methods<\/h3>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"140\" height=\"140\" data-id=\"1378\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2023\/10\/image-26.png\" alt=\"\" class=\"wp-image-1378\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2023\/10\/image-26.png 140w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2023\/10\/image-26-100x100.png 100w\" sizes=\"auto, (max-width: 140px) 100vw, 140px\" \/><figcaption class=\"wp-element-caption\">      Taobao store<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"396\" height=\"396\" data-id=\"823\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2023\/02\/\u5434\u7115.png\" alt=\"\" class=\"wp-image-823\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2023\/02\/\u5434\u7115.png 396w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2023\/02\/\u5434\u7115-300x300.png 300w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2023\/02\/\u5434\u7115-100x100.png 100w\" sizes=\"auto, (max-width: 396px) 100vw, 396px\" \/><figcaption class=\"wp-element-caption\">           Wechat\uff08TEL:18112558497)<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"900\" height=\"720\" data-id=\"1599\" src=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2024\/01\/R.jpg\" alt=\"\" class=\"wp-image-1599\" srcset=\"https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2024\/01\/R.jpg 900w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2024\/01\/R-600x480.jpg 600w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2024\/01\/R-768x614.jpg 768w, https:\/\/www.dxfluidics.com\/wp-content\/uploads\/2024\/01\/R-15x12.jpg 15w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><figcaption class=\"wp-element-caption\">   E-mail\uff1asale@dxfluidics.com<\/figcaption><\/figure>\n<\/figure>","protected":false},"excerpt":{"rendered":"<p>\u5728\u7ec6\u80de\u751f\u7269\u5b66\u4e0e\u5fae\u7eb3\u5236\u9020\u4ea4\u53c9\u9886\u57df\uff0c\u7ebf\u7c92\u4f53\u6742\u5408\u7ec6\u80de\uff08transmitochondrial\u00a0cybrids\uff09\u7684\u5236\u5907\u6280 [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":5107,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_crdt_document":"","footnotes":""},"categories":[5],"tags":[],"class_list":["post-5098","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-information"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>\u5fae\u6d41\u63a7\u7ebf\u7c92\u4f53\u6742\u5408\u7ec6\u80de\u5236\u5907\uff1a\u4ece\u82af\u7247\u52a0\u5de5\u5230\u4e34\u5e8a\u5e94\u7528\u7684\u521b\u65b0\u8def\u5f84 \u9876\u65ed\u5fae\u63a7<\/title>\n<meta name=\"description\" content=\"\u8be5\u6280\u672f\u4ee5\u5fae\u6d41\u63a7\u82af\u7247\u4e3a\u6838\u5fc3\uff0c\u7a81\u7834\u4f20\u7edf\u4f9d\u8d56\u65e0 PU \u57f9\u517b\u57fa\u7684\u9650\u5236\uff0c\u5b9e\u73b0 PU 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