{"id":4042,"date":"2025-05-15T16:30:00","date_gmt":"2025-05-15T08:30:00","guid":{"rendered":"https:\/\/www.dxfluidics.com\/?p=4042"},"modified":"2025-05-15T13:33:44","modified_gmt":"2025-05-15T05:33:44","slug":"%e5%be%ae%e6%b5%81%e4%bd%93%e6%9c%ba%e6%a2%b0%e7%a9%bf%e5%ad%94%e5%9c%a8%e5%b9%b2%e7%bb%86%e8%83%9e%e4%b8%ad%e7%9a%84%e5%ba%94%e7%94%a8","status":"publish","type":"post","link":"https:\/\/www.dxfluidics.com\/en\/technical-support\/information\/4042\/%e5%be%ae%e6%b5%81%e4%bd%93%e6%9c%ba%e6%a2%b0%e7%a9%bf%e5%ad%94%e5%9c%a8%e5%b9%b2%e7%bb%86%e8%83%9e%e4%b8%ad%e7%9a%84%e5%ba%94%e7%94%a8\/","title":{"rendered":"\u5fae\u6d41\u4f53\u673a\u68b0\u7a7f\u5b54\u5728\u5e72\u7ec6\u80de\u4e2d\u7684\u5e94\u7528"},"content":{"rendered":"

\u53c2\u8003\u6587\u732e\uff1aMicrofluidic Mechanoporation: Current Progress and Applications in Stem Cells – PMC<\/a><\/p>\n\n\n\n

1.\u00a0\u00a0\u00a0 \u57fa\u4e8e\u5fae\u6d41\u4f53\u7684\u673a\u68b0\u7a7f\u5b54<\/h2>\n\n\n\n


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1)    \u663e\u5fae\u6ce8\u5c04<\/h3>\n\n\n\n

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2)    \u5fae\/\u7eb3\u7c73\u9488\u9635\u5217<\/h3>\n\n\n\n

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Zhang \u7b49\u4eba\u5f00\u53d1\u7684\u5fae\u6d41\u63a7\u5fae\u9488\u88c5\u7f6e\u96c6\u6210\u4e86\u5927\u89c4\u6a21\u5e76\u884c\u5fae\u6ce8\u5c04\u5668\u9635\u5217\uff08100\u00d7100\u4e2a\u6355\u83b7\u4f4d\u70b9\uff09\uff0c\u901a\u8fc7\u8d1f\u538b\u5438\u9644\u4f7f\u7ec6\u80de\u5438\u9644\u81f3\u5e26\u6709\u4e9a\u5fae\u7c73\u5c16\u7aef\u7684\u7a7a\u5fc3\u7a7f\u900f\u5668\u5e76\u5b8c\u6210\u7a7f\u819c\u6ce8\u5c04\uff0c\u968f\u540e\u6b63\u538b\u91ca\u653e\u7ec6\u80de\uff0c\u5b9e\u73b0\u8d85\u9ad8\u901a\u91cf\u548c\u9ad8\u6548\u7387\u7684\u8f6c\u67d3\uff0c\u5728T\u7ec6\u80de\u4e2d\u83b7\u5f97\u9ad8\u8fbe93%\u7684\u67d3\u6599\u8f6c\u67d3\u6548\u7387\u548c82%\u7684\u8d28\u7c92\u9012\u9001\u6548\u7387\uff0c\u7ec6\u80de\u6d3b\u529b\u8d85\u8fc787%\u3002<\/p>\n\n\n\n

Huang \u7b49\u4eba\u8bbe\u8ba1\u7684\u5fae\u6d41\u63a7\u7eb3\u7c73\u9488\u88c5\u7f6e\u7ed3\u5408\u7845\u7eb3\u7c73\u9488\u9635\u5217\u4e0e\u4eba\u5b57\u5f62\u901a\u9053\uff0c\u901a\u8fc7\u7eb3\u7c73\u9488\u4e0e\u7ec6\u80de\u63a5\u89e6\u5f62\u6210\u5b54\u9699\uff0c\u4f7f\u5916\u6e90\u7269\u8d28\u6269\u6563\u8fdb\u5165\u7ec6\u80de\uff0c\u5b9e\u9a8c\u4e2d\u5728\u4eba\u80da\u80be\u7ec6\u80de\u4e2d\u83b7\u5f97\u8d85\u8fc720%\u7684GFP\u8d28\u7c92\u8f6c\u67d3\u6548\u7387\u548c95%\u4ee5\u4e0a\u7684\u7ec6\u80de\u6d3b\u529b\u3002<\/p>\n\n\n

\n
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3)    \u57fa\u4e8e\u673a\u68b0\u9650\u5236\u7684\u7ec6\u80de\u6324\u538b<\/h3>\n\n\n\n

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\n
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\u57fa\u4e8e\u673a\u68b0\u9650\u5236\u7684\u7ec6\u80de\u6324\u538b<\/figcaption><\/figure>\n<\/div>\n\n\n

4)    \u57fa\u4e8e\u6d41\u4f53\u52a8\u529b\u5b66\u64cd\u63a7\u7684\u7ec6\u80de\u6324\u538b<\/h3>\n\n\n\n

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\u8be5\u5e73\u53f0\u6210\u529f\u5c06 siRNA \u8f93\u9001\u81f3 HeLa \u7ec6\u80de\uff0c\u5e76\u5b9e\u73b0\u9ad8\u8fbe 97% \u7684 ITGA1 \u57fa\u56e0\u6572\u4f4e\u7387\uff0c\u5c55\u73b0\u51fa\u5176\u5728\u57fa\u56e0\u7f16\u8f91\u4e2d\u7684\u5e94\u7528\u6f5c\u529b\u3002<\/p>\n\n\n

\n
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\u57fa\u4e8e\u6d41\u4f53\u52a8\u529b\u5b66\u64cd\u63a7\u7684\u7ec6\u80de\u6324\u538b<\/figcaption><\/figure>\n<\/div>\n\n\n

2.    \u673a\u68b0\u7a7f\u5b54\u5728\u5e72\u7ec6\u80de\u4e2d\u7684\u5e94\u7528<\/h2>\n\n\n\n

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\u81ea 1998 \u5e74\u4eba\u7c7b\u80da\u80ce\u5e72\u7ec6\u80de\uff08hESC\uff09\u9996\u6b21\u5206\u79bb\u4ee5\u6765\uff0c\u8bf1\u5bfc\u591a\u80fd\u5e72\u7ec6\u80de\uff08iPSC\uff09\u7b49\u6210\u4f53\u5e72\u7ec6\u80de\u6a21\u578b\u4e5f\u88ab\u5e7f\u6cdb\u7528\u4e8e\u7814\u7a76\u3002\u5e72\u7ec6\u80de\u7597\u6cd5\u6709\u671b\u7528\u4e8e\u6cbb\u7597\u5fc3\u810f\u75c5\u3001I \u578b\u7cd6\u5c3f\u75c5\u7b49\u75be\u75c5\u3002<\/p>\n\n\n\n

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1)    \u5e72\u7ec6\u80de\u4e0d\u540c\u8f6c\u67d3\u65b9\u6cd5\u7684\u6bd4\u8f83<\/h3>\n\n\n\n

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\n
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2)    \u673a\u68b0\u7a7f\u5b54\u6280\u672f\u5728\u5e72\u7ec6\u80de\u4e2d\u7684\u5e94\u7528<\/h3>\n\n\n\n

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\n
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