{"id":4013,"date":"2025-05-08T16:30:00","date_gmt":"2025-05-08T08:30:00","guid":{"rendered":"https:\/\/www.dxfluidics.com\/?p=4013"},"modified":"2025-05-08T13:57:32","modified_gmt":"2025-05-08T05:57:32","slug":"%e5%8d%95%e7%bb%86%e8%83%9erna%e6%b5%8b%e5%ba%8f%e6%8a%80%e6%9c%af","status":"publish","type":"post","link":"https:\/\/www.dxfluidics.com\/en\/technical-support\/information\/4013\/%e5%8d%95%e7%bb%86%e8%83%9erna%e6%b5%8b%e5%ba%8f%e6%8a%80%e6%9c%af\/","title":{"rendered":"\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6280\u672f"},"content":{"rendered":"

\u53c2\u8003\u6587\u732e\uff1aAdvances in Microfluidic Single-Cell RNA Sequencing and Spatial Transcriptomics – PMC<\/a><\/p>\n\n\n\n

1.    \u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6280\u672f\u7684\u8fdb\u5c55<\/h2>\n\n\n\n

\u5355\u7ec6\u80de\u6d4b\u5e8f\uff08SCS\uff09\u65b9\u6cd5\u4e3b\u8981\u5305\u62ecmicroplate<\/a>\u548c\u5fae\u6d41\u63a7<\/a>\u4e24\u7c7b\uff0c\u5176\u4e2d\u4ee5Smart-seq2\u4e3a\u4ee3\u8868\u7684\u5fae\u5b54\u677f\u65b9\u6cd5\u53ef\u83b7\u53d6\u5168\u957f\u8f6c\u5f55\u672c\uff0c\u4f46\u901a\u91cf\u53d7\u9650\uff1b<\/p>\n\n\n\n

\u800c\u5fae\u6d41\u63a7\u65b9\u6cd5\u901a\u8fc7\u5c06\u7ec6\u80de\u5206\u9694\u81f3\u7eb3\u5347\u7ea7\u53cd\u5e94\u8154\u4e2d\uff0c\u663e\u8457\u964d\u4f4e\u4ea4\u53c9\u6c61\u67d3\uff0c\u5e76\u5177\u5907\u9ad8\u901a\u91cf\u3001\u53ef\u5b9a\u5236\u6027\u5f3a\u7b49\u4f18\u52bf\u3002<\/p>\n\n\n\n

\u5c24\u5176\u5728\u5fae\u6d41\u63a7\u88c5\u7f6e\u4e0e\u9ad8\u5bc6\u5ea6\u5fae\u5b54\u9635\u5217\u7684\u652f\u6301\u4e0b\uff0cSCS\u4e0d\u4ec5\u63d0\u5347\u4e86\u6570\u636e\u4e00\u81f4\u6027\u4e0e\u5904\u7406\u6548\u7387\uff0c\u8fd8\u4e3a\u591a\u6837\u5316\u751f\u7269\u6837\u672c\u63d0\u4f9b\u4e86\u53ef\u6269\u5c55\u7684\u5206\u6790\u5e73\u53f0\u3002<\/p>\n\n\n\n

1)    \u57fa\u4e8e\u6db2\u6ef4\u7684\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u65b9\u6cd5<\/h3>\n\n\n\n

\u6db2\u6ef4\u5fae\u6d41\u63a7\u7cfb\u7edf<\/a>\u901a\u8fc7\u5c06\u7ec6\u80de\u60ac\u6db2\u5206\u5272\u6210\u7531\u6cb9\u76f8\u5305\u88f9\u7684\u6c34\u76f8\u6db2\u6ef4\uff0c\u5b9e\u73b0\u5355\u7ec6\u80de\u7684\u9ad8\u901a\u91cf\u5c01\u88c5\u4e0e\u8f6c\u5f55\u672c\u6355\u83b7\uff0c\u76ee\u524d\u7684\u6db2\u6ef4\u5f0fscRNA-seq\u6d41\u7a0b\u4e3b\u8981\u5305\u62ec\u7ec6\u80de\u5bcc\u96c6\u3001\u6db2\u6ef4\u5c01\u88c5\u3001\u9006\u8f6c\u5f55\u53ca\u6269\u589e\u3001\u6d4b\u5e8f\u548c\u6570\u636e\u5206\u6790\u4e94\u4e2a\u6b65\u9aa4\u3002<\/p>\n\n\n\n

\u4ee3\u8868\u6027\u65b9\u6cd5Drop-seq\u548cinDrop\u867d\u5728\u6761\u5f62\u7801\u6807\u8bb0\u539f\u7406\u4e0a\u76f8\u540c\uff0c\u4f46\u5728\u5c01\u88c5\u65b9\u5f0f\u3001\u9006\u8f6c\u5f55\u73af\u5883\u53ca\u6269\u589e\u7b56\u7565\u4e0a\u5b58\u5728\u5dee\u5f02\uff0c\u524d\u8005\u4f7f\u7528\u521a\u6027\u73e0\u5b50\u7edf\u4e00RT\u53cd\u5e94\u5e76\u57fa\u4e8eSmart-Seq\u7684PCR\u6269\u589e\uff0c\u7075\u654f\u5ea6\u9ad8\u4f46\u504f\u501a\u660e\u663e\uff1b<\/p>\n\n\n\n

\u540e\u8005\u5229\u7528\u6761\u7801\u5316\u6c34\u51dd\u80f6\u5fae\u7403\u5206\u522b\u8fdb\u884cRT\u53cd\u5e94\u5e76\u91c7\u7528CEL-Seq\u7684\u7ebf\u6027IVT\u6269\u589e\uff0c\u6269\u589e\u504f\u5dee\u66f4\u4f4e\u3002<\/p>\n\n\n

\n
\"\"
Drop-seq \u6280\u672f\u5de5\u4f5c\u6d41\u7a0b\u793a\u610f\u56fe<\/figcaption><\/figure>\n<\/div>\n\n\n

2)    \u57fa\u4e8e\u5fae\u5b54\u9635\u5217\u7684\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u65b9\u6cd5<\/h3>\n\n\n\n

\u76f8\u6bd4\u4e8e\u6db2\u6ef4\u5fae\u6d41\u63a7\u6280\u672f\uff0c\u57fa\u4e8e\u5fae\u5b54\u9635\u5217\u7684scRNA-seq\u65b9\u6cd5\u51ed\u501f\u5176\u7ed3\u6784\u7edf\u4e00\u3001\u64cd\u4f5c\u7b80\u4fbf\u548c\u6210\u672c\u6548\u76ca\u9ad8\u7b49\u4f18\u52bf\uff0c\u53ef\u5728\u6574\u4f53\u6c34\u5e73\u4e0a\u5b9e\u73b0\u66f4\u65e0\u504f\u7684\u5355\u7ec6\u80de\u6d4b\u5e8f\u3002<\/p>\n\n\n\n

\u6b64\u7c7b\u65b9\u6cd5\u901a\u8fc7\u7eb3\u5347\u7ea7\u6216\u76ae\u5347\u7ea7\u5fae\u5b54\u4f5c\u4e3a\u72ec\u7acb\u53cd\u5e94\u5668\uff0c\u5229\u7528\u91cd\u529b\u88c5\u8f7d\u5355\u7ec6\u80de\uff0c\u5e76\u5728\u539f\u4f4d\u5b8c\u6210\u88c2\u89e3\u3001RNA\u6355\u83b7<\/a>\u548c\u9006\u8f6c\u5f55\u3002<\/p>\n\n\n\n

\u4ee3\u8868\u6027\u6280\u672f\u5982Cytoseq\u3001Microwell-seq\u548cSeq-Well\u7b49\uff0c\u901a\u8fc7\u7075\u6d3b\u7684\u5c01\u95ed\u7b56\u7565\uff08\u5982\u673a\u68b0\u5bc6\u5c01\u3001\u6cb9\u5c01\u95ed\u6216\u534a\u900f\u819c\uff09\u6709\u6548\u964d\u4f4e\u4ea4\u53c9\u6c61\u67d3\uff0c\u540c\u65f6\u7ed3\u5408\u56fa\u76f8\u6355\u83b7\u3001\u78c1\u73e0\u6761\u7801\u5316\u548c\u7b80\u5316\u6587\u5e93\u6784\u5efa\u6d41\u7a0b\uff0c\u63a8\u52a8\u4e86\u5176\u5728\u514d\u75ab\u5b66\u3001\u795e\u7ecf\u79d1\u5b66\u7b49\u9886\u57df\u7684\u5e94\u7528\u3002<\/p>\n\n\n

\n
\"\"
CytoSeq \u7684\u793a\u610f\u56fe\u548c\u56fe\u50cf\u6570\u636e<\/figcaption><\/figure>\n<\/div>\n\n\n

3)    \u57fa\u4e8e\u9600\u95e8\u7684\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u65b9\u6cd5<\/h3>\n\n\n\n

\u57fa\u4e8e\u9600\u95e8\u7684\u5fae\u6d41\u63a7\u82af\u7247\u901a\u8fc7\u8c03\u8282\u538b\u529b\u63a7\u5236\u5f39\u6027PDMS\u819c<\/a>\u9600\u95e8\u7684\u5f00\u5408\uff0c\u5b9e\u73b0\u5bf9\u5355\u7ec6\u80de\u7684\u7cbe\u51c6\u5206\u79bb<\/a>\u3001\u6355\u83b7\u4e0e\u5904\u7406\uff0c\u5177\u5907\u9ad8\u7075\u654f\u5ea6\u3001\u9ad8\u901a\u91cf\u548c\u4f4e\u6c61\u67d3\u7b49\u4f18\u52bf\u3002<\/p>\n\n\n\n

\u4ee3\u8868\u6027\u5e73\u53f0\u5982Streets\u56e2\u961f\u7684\u81ea\u52a8\u5316\u82af\u7247\u3001Hydro-Seq\u53caPaired-seq\u7b49\uff0c\u901a\u8fc7\u4f18\u5316\u9600\u95e8\u8bbe\u8ba1\u548c\u901a\u9053\u7ed3\u6784\uff0c\u53ef\u6709\u6548\u6392\u9664\u6742\u7ec6\u80de\u53ca\u6e38\u79bbRNA\uff0c\u9002\u7528\u4e8e\u5faa\u73af\u80bf\u7624\u7ec6\u80de\uff08CTCs\uff09\u7b49\u73cd\u8d35\u6837\u672c\u7684\u7eaf\u5316\u4e0e\u5206\u6790\u3002<\/p>\n\n\n\n

\u540c\u65f6\uff0c\u5546\u7528\u5e73\u53f0Fluidigm C1\u652f\u6301\u591a\u79cd\u6587\u5e93\u6784\u5efa\u534f\u8bae\uff0c\u80fd\u591f\u83b7\u53d6\u5168\u957f\u8f6c\u5f55\u672c\uff0c\u4fbf\u4e8e\u5728\u4e34\u5e8a\u6837\u672c\u4e2d\u8fdb\u884c\u9ad8\u8d28\u91cf\u8868\u8fbe\u8c31\u5206\u6790\u3002<\/p>\n\n\n

\n
\"\"
\u5fae\u6d41\u63a7\u88c5\u7f6e\u7684\u663e\u5fae\u7167\u7247<\/figcaption><\/figure>\n<\/div>\n\n\n

4)    \u5355\u7ec6\u80de\u591a\u7ec4\u5b66\u5206\u6790<\/h3>\n\n\n\n

\u751f\u7269\u5b66\u4e2d\u5fc3\u6cd5\u5219\u63ed\u793a\u4e86DNA\u3001RNA\u4e0e\u86cb\u767d\u8d28\u4e4b\u95f4\u7684\u76f8\u4e92\u5173\u7cfb\uff0c\u5355\u4e00\u7ec4\u5b66\u96be\u4ee5\u5168\u9762\u53cd\u6620\u7ec6\u80de\u529f\u80fd\u4e0e\u72b6\u6001\uff0c\u56e0\u6b64\u5355\u7ec6\u80de\u591a\u7ec4\u5b66\u6280\u672f\u5e94\u8fd0\u800c\u751f\uff0c\u4ee5\u540c\u65f6\u6355\u6349\u7ec6\u80de\u5728\u65f6\u95f4\u548c\u7a7a\u95f4\u7ef4\u5ea6\u4e0a\u7684\u52a8\u6001\u53d8\u5316\u3002<\/p>\n\n\n\n

\u5fae\u6d41\u63a7\u5e73\u53f0\u51ed\u501f\u5176\u5bf9\u5355\u7ec6\u80de\u7cbe\u786e\u64cd\u63a7\u7684\u80fd\u529b\uff0c\u6210\u4e3a\u6574\u5408\u8f6c\u5f55\u7ec4\u3001\u86cb\u767d\u8d28\u7ec4\u3001\u57fa\u56e0\u7ec4\u4e43\u81f3\u8868\u89c2\u7ec4\u5b66\u7684\u7406\u60f3\u5de5\u5177\u3002<\/p>\n\n\n\n

\u5982CITE-seq\u548cREAP-seq\u53ef\u540c\u65f6\u8bfb\u53d6\u8f6c\u5f55\u672c\u4e0e\u86cb\u767d\u8868\u8fbe\u4fe1\u606f\uff0c\u9002\u914d\u4f53\u66ff\u4ee3\u6297\u4f53\u8fdb\u4e00\u6b65\u964d\u4f4e\u4e86\u6210\u672c\uff1bASAP-seq\u4e0eNEAT-seq\u5219\u5b9e\u73b0\u4e86\u5bf9\u67d3\u8272\u8d28\u53ef\u53ca\u6027\u3001\u8f6c\u5f55\u672c\u548c\u86cb\u767d\u7684\u4e09\u91cd\u68c0\u6d4b\u3002<\/p>\n\n\n\n

\u901a\u8fc7\u96c6\u6210\u591a\u5c42\u6b21\u6570\u636e\uff0c\u8fd9\u4e9b\u65b9\u6cd5\u4e0d\u4ec5\u80fd\u63ed\u793a\u7ec6\u80de\u5f02\u8d28\u6027\u3001\u75be\u75c5\u8fdb\u7a0b\u53ca\u514d\u75ab\u8c03\u63a7\u673a\u5236\uff0c\u8fd8\u4e3a\u7b5b\u9009\u6f5c\u5728\u75be\u75c5\u6807\u5fd7\u7269\u4e0e\u5173\u952e\u8c03\u63a7\u56e0\u5b50\u63d0\u4f9b\u4e86\u5168\u65b0\u8def\u5f84\u3002<\/p>\n\n\n

\n
\"\"
CITE-seq \u4e2d\u4f7f\u7528\u7684 DNA \u6761\u5f62\u7801\u6297\u4f53\u4ee5\u53ca\u5c0f\u9f20\u548c\u4eba\u7c7b\u7ec6\u80de\u6df7\u5408\u7269\u5206\u6790\u7684\u56fe\u793a<\/figcaption><\/figure>\n<\/div>\n\n\n

2.    \u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u7684\u8fdb\u5c55<\/h2>\n\n\n\n

\u5c3d\u7ba1\u5355\u7ec6\u80de\u6d4b\u5e8f\uff08SCS\uff09\u80fd\u591f\u63ed\u793a\u7ec6\u80de\u95f4\u5f02\u8d28\u6027\uff0c\u4f46\u65e0\u6cd5\u89e3\u6790\u7ec6\u80de\u95f4\u53ca\u5176\u4e0e\u5fae\u73af\u5883\u7684\u7a7a\u95f4\u5173\u7cfb\u3002<\/p>\n\n\n\n

\u7a7a\u95f4\u5206\u8fa8\u8f6c\u5f55\u7ec4\u5b66\uff08SRT\uff09\u6280\u672f\u901a\u8fc7\u6210\u50cf\u3001\u6fc0\u5149\u663e\u5fae\u5207\u5272\uff08LCM\uff09\u548c\u6d4b\u5e8f\u4e09\u7c7b\u65b9\u6cd5\uff0c\u4e3a\u7814\u7a76\u7ec4\u7ec7\u53d1\u80b2\u3001\u7a33\u6001\u4e0e\u75be\u75c5\u673a\u5236\u63d0\u4f9b\u4e86\u65b0\u7684\u89c6\u89d2\u3002<\/p>\n\n\n\n

\u5176\u4e2d\uff0c\u6d4b\u5e8f\u7c7bSRT\u56e0\u5177\u5907\u975e\u504f\u501a\u3001\u9ad8\u901a\u91cf\u548c\u4f4e\u9519\u8bef\u7387\u7b49\u4f18\u52bf\u800c\u88ab\u5e7f\u6cdb\u5e94\u7528\u3002<\/p>\n\n\n\n

1)    \u57fa\u4e8e\u9635\u5217\u7684\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\uff08SRT\uff09\u65b9\u6cd5<\/h3>\n\n\n\n

\u57fa\u4e8e\u9635\u5217\u7684\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\u6280\u672f\u901a\u8fc7\u8868\u9762\u6216\u5fae\u73e0\u6355\u83b7polyA RNA\uff0c\u5728\u4fdd\u7559\u7a7a\u95f4\u4fe1\u606f\u7684\u540c\u65f6\u5b9e\u73b0\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u7ec4\u7ec7\u5f02\u8d28\u6027\u7814\u7a76\u3002<\/p>\n\n\n\n

\u8868\u9762\u6355\u83b7\u65b9\u6cd5\u5982ST\u6280\u672f\u548c\u5176\u5546\u4e1a\u5316\u5e73\u53f0Visium\uff0c\u901a\u8fc7\u5728\u73bb\u7483\u7247\u4e0a\u4fee\u9970\u6355\u83b7\u63a2\u9488\u5e76\u8d4b\u4e88\u7a7a\u95f4\u6761\u5f62\u7801\uff0c\u5df2\u5b9e\u73b0\u4ece200\u5fae\u7c73\u81f3100\u5fae\u7c73\u7684\u5206\u8fa8\u7387\u63d0\u5347\uff0c\u540e\u7eed\u5982SM-Omics\u4e0eSPOTS\u66f4\u6574\u5408\u86cb\u767d\u5206\u6790\u4ee5\u589e\u5f3a\u591a\u7ec4\u5b66\u80fd\u529b\u3002<\/p>\n\n\n\n

\u5fae\u73e0\u6355\u83b7\u65b9\u6cd5\u5982Slide-Seq\u548cHDST\u5219\u4f7f\u7528\u76f4\u5f84\u5206\u522b\u4e3a10\u5fae\u7c73\u548c2\u5fae\u7c73\u7684\u78c1\u6027\u5fae\u73e0\u6784\u5efa\u9ad8\u5bc6\u5ea6\u9635\u5217\uff0c\u867d\u5177\u5907\u8fd1\u5355\u7ec6\u80de\u7ea7\u5206\u8fa8\u7387\uff0c\u4f46\u7531\u4e8e\u6355\u83b7\u6548\u7387\u4e0d\u8db3\u53ca\u6761\u5f62\u7801\u6620\u5c04\u5230\u76f8\u90bb\u5207\u7247\u800c\u975e\u5f53\u524d\u7ec4\u7ec7\uff0c\u4ecd\u9762\u4e34\u6570\u636e\u4e0d\u8db3\u548c\u5b9a\u4f4d\u504f\u5dee\u7b49\u6311\u6218\uff0c\u7279\u522b\u5728\u5904\u7406\u80bf\u7624\u7b49\u9ad8\u5ea6\u5f02\u8d28\u6027\u6837\u672c\u65f6\u9650\u5236\u660e\u663e\u3002<\/p>\n\n\n

\n
\"\"
\u7a7a\u95f4\u5b9a\u4f4d cDNA \u5408\u6210<\/figcaption><\/figure>\n<\/div>\n\n\n

2)    \u57fa\u4e8e\u5fae\u901a\u9053\u7684\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\uff08SRT\uff09\u65b9\u6cd5<\/h3>\n\n\n\n

\u57fa\u4e8e\u5fae\u901a\u9053\u7684\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u5b66\uff08SRT\uff09\u65b9\u6cd5\u4ee5\u5176\u53ef\u8c03\u8282\u7684\u7a7a\u95f4\u6761\u5f62\u7801\u5206\u8fa8\u7387\u548c\u5f3a\u5927\u7684\u591a\u7ec4\u5b66\u96c6\u6210\u80fd\u529b\uff0c\u4e3a\u7ec4\u7ec7\u5185\u751f\u7269\u8c03\u63a7\u7f51\u7edc\u7684\u7cbe\u7ec6\u89e3\u6790\u63d0\u4f9b\u4e86\u65b0\u89c6\u89d2\u3002<\/p>\n\n\n\n

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\u8be5\u65b9\u6cd5\u4e0d\u4ec5\u9002\u7528\u4e8e\u5f62\u6001\u7ed3\u6784\u5bf9\u9f50\u7684\u5149\u5b66\u6210\u50cf\u5206\u6790\uff0c\u8fd8\u53ef\u901a\u8fc7\u4fee\u9970\u987a\u78c1\u9897\u7c92\u4f18\u5316\u901a\u9053\u7a33\u5b9a\u6027\uff0c\u63d0\u5347\u7ec4\u7ec7\u9002\u5e94\u6027\u3002<\/p>\n\n\n\n

\u56e0\u6b64\uff0c\u4ee5DBiT-seq\u4e3a\u4ee3\u8868\u7684\u5fae\u901a\u9053SRT\u7b56\u7565\u5177\u5907\u826f\u597d\u7684\u901a\u7528\u6027\u548c\u53ef\u6269\u5c55\u6027\uff0c\u4e3a\u591a\u7ef4\u7a7a\u95f4\u751f\u7269\u4fe1\u606f\u7684\u9ad8\u7cbe\u5ea6\u6355\u83b7\u63d0\u4f9b\u4e86\u5f3a\u5927\u652f\u6301\u3002<\/p>\n\n\n

\n
\"\"
DBiT-seq \u4e2d\u4f7f\u7528\u7684\u5fae\u6d41\u4f53\u88c5\u7f6e<\/figcaption><\/figure>\n<\/div>\n\n\n

3)    \u65f6\u7a7a\u8f6c\u5f55\u7ec4<\/h3>\n\n\n\n

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\u8bf8\u5982Slide-seqV2\u548cStereo-seq\u7b49\u5148\u8fdb\u5e73\u53f0\u5df2\u6210\u529f\u6784\u5efa\u4e86\u5c0f\u9f20\u5927\u8111\u4e0e\u773c\u90e8\u7b49\u5668\u5b98\u7684\u53d1\u80b2\u8f68\u8ff9\uff0c\u751a\u81f3\u5b9e\u73b0\u4e86\u4eba\u8111\u8de8\u65f6\u95f4\u70b9\u7684\u7a7a\u95f4\u53d1\u80b2\u56fe\u8c31\u63cf\u7ed8\u3002<\/p>\n\n\n\n

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\n
\"\"
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