{"id":3993,"date":"2025-04-29T16:30:00","date_gmt":"2025-04-29T08:30:00","guid":{"rendered":"https:\/\/www.dxfluidics.com\/?p=3993"},"modified":"2025-04-30T08:36:38","modified_gmt":"2025-04-30T00:36:38","slug":"%e5%b7%a5%e7%a8%8b%e8%82%9d%e8%84%8f%e7%b1%bb%e5%99%a8%e5%ae%98%e6%8a%80%e6%9c%af","status":"publish","type":"post","link":"https:\/\/www.dxfluidics.com\/en\/technical-support\/information\/3993\/%e5%b7%a5%e7%a8%8b%e8%82%9d%e8%84%8f%e7%b1%bb%e5%99%a8%e5%ae%98%e6%8a%80%e6%9c%af\/","title":{"rendered":"\u5de5\u7a0b\u809d\u810f\u7c7b\u5668\u5b98\u6280\u672f"},"content":{"rendered":"

\u53c2\u8003\u6587\u732e\uff1aEmerging biotechnologies for engineering liver organoids – PMC<\/a><\/p>\n\n\n\n

\u809d\u810f\u7c7b\u5668\u5b98<\/a>\u901a\u8fc7\u5f62\u6210\u7ed3\u6784\u6709\u5e8f\u3001\u529f\u80fd\u591a\u6837\u76843D\u7ed3\u6784<\/a>\uff0c\u514b\u670d\u4e86\u4f20\u7edf2D\u57f9\u517b\u548c\u52a8\u7269\u6a21\u578b\u7684\u5c40\u9650\uff0c\u6210\u4e3a\u75be\u75c5\u5efa\u6a21\u3001drug screening<\/a>\u53ca\u7ec4\u7ec7\u5de5\u7a0b\u7684\u91cd\u8981\u5de5\u5177\u3002<\/p>\n\n\n\n

\u5176\u5229\u7528\u539f\u4ee3\u809d\u7ec6\u80de\u6216iPSCs\u5728Matrigel\u7b49\u652f\u67b6\u4e2d\u57f9\u517b\uff0c\u4f46\u9762\u4e34\u53d8\u5f02\u6027\u5927\u3001\u6210\u719f\u5ea6\u4e0d\u8db3\u7b49\u95ee\u9898\u3002<\/p>\n\n\n\n

\u5de5\u7a0b\u5316\u89e3\u51b3\u65b9\u6848\u91c7\u7528\u5929\u7136\/\u5408\u6210\u6c34\u51dd\u80f6\u6216\u8131\u7ec6\u80de\u652f\u67b6\uff0c\u7ed3\u5408\u5fae\u6d41\u63a7\u30013D\u751f\u7269\u6253\u5370\u7b49\u6280\u672f\u63d0\u5347\u53ef\u63a7\u6027\u4e0e\u4e00\u81f4\u6027\uff0c\u800c\u57fa\u56e0\u5de5\u7a0b\u8fdb\u4e00\u6b65\u901a\u8fc7\u6539\u9020\u5e72\u7ec6\u80de\u5b9a\u5236\u7c7b\u5668\u5b98\u529f\u80fd\uff0c\u63a8\u52a8\u5176\u5411\u66f4\u590d\u6742\u3001\u66f4\u4eff\u751f\u7684\u65b9\u5411\u53d1\u5c55\u3002<\/p>\n\n\n\n

1.    \u5de5\u7a0b\u5316\u809d\u810f\u7c7b\u5668\u5b98\u7684\u57fa\u8d28\u6750\u6599<\/h2>\n\n\n\n

\u7ec6\u80de\u5916\u57fa\u8d28\uff08ECM\uff09\u53ca\u5176\u6a21\u62df\u7269\u5728\u809d\u7c7b\u5668\u5b98\u57f9\u517b\u4e2d\u53d1\u6325\u5173\u952e\u4f5c\u7528\uff0c\u63d0\u4f9b\u4e09\u7ef4\u652f\u6491\u5e76\u8c03\u63a7\u7ec6\u80de\u884c\u4e3a\u3002<\/p>\n\n\n\n

\u5929\u7136\u6c34\u51dd\u80f6\uff08\u5982Matrigel\u3001\u80f6\u539f\u86cb\u767d\u3001\u58f3\u805a\u7cd6\u7b49\uff09\u5177\u6709\u826f\u597d\u751f\u7269\u6d3b\u6027\u4f46\u5b58\u5728\u5f02\u8d28\u6027\uff0c\u800c\u6539\u6027<\/a>\u6750\u6599\uff08\u5982GelMA\uff09\u901a\u8fc7\u53ef\u8c03\u6027\u80fd\u4f18\u5316\u57f9\u517b\u73af\u5883\u3002<\/p>\n\n\n\n

\u5408\u6210\u6c34\u51dd\u80f6\uff08\u5982PEG\u3001PIC\uff09\u53ef\u7cbe\u51c6\u8c03\u63a7\u529b\u5b66\u548c\u751f\u5316\u7279\u6027\uff0c\u7ecfRGD\u6216\u5c42\u7c98\u8fde\u86cb\u767d\u4fee\u9970\u540e\u80fd\u5ab2\u7f8e\u751a\u81f3\u8d85\u8d8aMatrigel\u3002<\/p>\n\n\n\n

\u8131\u7ec6\u80de\u652f\u67b6\u4fdd\u7559\u5929\u7136ECM\u7684\u5b8c\u6574\u7ed3\u6784\u548c\u751f\u7269\u4fe1\u53f7\uff0c\u652f\u6301\u590d\u6742\u7c7b\u5668\u5b98\u6784\u5efa\uff0c\u4f46\u9700\u89e3\u51b3\u6279\u6b21\u53d8\u5f02\u548c\u514d\u75ab\u539f\u6027\u95ee\u9898\u3002<\/p>\n\n\n\n

2.    \u5de5\u7a0b\u5316\u6280\u672f\u5728\u809d\u810f\u7c7b\u5668\u5b98\u4e2d\u7684\u5e94\u7528<\/h2>\n\n\n\n

\u4f20\u7edf\u7c7b\u5668\u5b98\u57f9\u517b\u65b9\u6cd5\uff08\u5982Matrigel\u7a79\u9876\u57f9\u517b\u3001\u60ac\u6ef4\u57f9\u517b\u7b49\uff09\u5b58\u5728\u64cd\u4f5c\u7e41\u7410\u3001\u6279\u6b21\u5dee\u5f02\u5927\u7684\u5c40\u9650\u6027\u3002<\/p>\n\n\n

\n
\"\"
\u7c7b\u5668\u5b98\u5236\u5907\u7b56\u7565<\/figcaption><\/figure>\n<\/div>\n\n\n

\u4e3a\u7a81\u7834\u8fd9\u4e9b\u74f6\u9888\uff0c\u65b0\u5174\u5de5\u7a0b\u5316\u6280\u672f\u901a\u8fc7\u81ea\u52a8\u5316\u4e0e\u6807\u51c6\u5316\u5de5\u827a\uff0c\u663e\u8457\u63d0\u5347\u4e86\u7c7b\u5668\u5b98\u751f\u4ea7\u7684\u89c4\u6a21\u5316\u548c\u5747\u4e00\u6027\u3002<\/p>\n\n\n\n

1)    \u6db2\u6ef4\u5fae\u6d41\u63a7\u6280\u672f<\/h3>\n\n\n\n

Droplet Microfluidics<\/a>\u901a\u8fc7\u8c03\u63a7\u6d41\u4f53\u53c2\u6570\u53ef\u9ad8\u6548\u5236\u5907\u5c3a\u5bf8\u5747\u4e00\u7684\u6c34\u51dd\u80f6\u5fae\u80f6\u56ca\uff0c\u4e3a\u809d\u7c7b\u5668\u5b98\u57f9\u517b\u63d0\u4f9b\u7406\u60f33D\u5e73\u53f0\u3002<\/p>\n\n\n

\n
\"\"
\u6db2\u6ef4\u5fae\u6d41\u63a7\u6280\u672f\u5728\u809d\u810f\u7c7b\u5668\u5b98\u5236\u5907\u4e2d\u7684\u5e94\u7528<\/figcaption><\/figure>\n<\/div>\n\n\n

\u4f8b\u5982\uff0c\u5c06hiPSC\u6765\u6e90\u7684\u809d\u524d\u4f53\u7ec6\u80de\u4e0e\u652f\u6301\u7ec6\u80de\u5c01\u88c5\u5728\u53ef\u964d\u89e3\u80bd\u57fa\u6c34\u51dd\u80f6\u4e2d\uff0c\u53ef\u81ea\u7ec4\u88c5\u5f62\u6210\u5177\u6709\u80c6\u7ba1\u548c\u8840\u7ba1\u7f51\u7edc\u7684\u590d\u6742\u809d\u7c7b\u5668\u5b98\u3002<\/p>\n\n\n\n

\u8be5\u6280\u672f\u5b9e\u73b0\u4e86\u7c7b\u5668\u5b98\u7684\u89c4\u6a21\u5316\u3001\u6807\u51c6\u5316\u751f\u4ea7\uff0c\u514b\u670d\u4e86\u4f20\u7edf\u65b9\u6cd5\u7684\u53ef\u63a7\u6027\u4e0e\u53ef\u91cd\u590d\u6027\u96be\u9898\u3002<\/p>\n\n\n\n

2)    \u58f0\u5b66\u6d41\u4f53\u5b66<\/h3>\n\n\n\n

\u58f0\u5b66\u6d41\u4f53\u6280\u672f<\/a>\u5229\u7528\u58f0\u573a\u5feb\u901f\u8bf1\u5bfc\u7ec6\u80de\u805a\u96c6\uff0c\u76f8\u6bd4\u4f20\u7edf\u65b9\u6cd5\u663e\u8457\u63d0\u5347\u6548\u7387\u3002<\/p>\n\n\n

\n
\"\"
\u58f0\u5b66\u6d41\u4f53\u6280\u672f\u5728\u7ec6\u80de\u805a\u96c6\u4f53\u5236\u5907\u4e2d\u7684\u5e94\u7528<\/figcaption><\/figure>\n<\/div>\n\n\n

\u901a\u8fc7\u80f6\u539f\u86cb\u767d\u8f85\u52a9\u6216\u538b\u7535\u6362\u80fd\u5668\u9635\u5217\uff0c\u53ef\u5728\u79d2\u7ea7\u5f62\u6210\u7a33\u5b9a\u7ec6\u80de\u805a\u96c6\u4f53\uff0c\u5e76\u6210\u529f\u5e94\u7528\u4e8e\u4e73\u817a\u80bf\u7624\u548c\u809d\u7c7b\u5668\u5b98\u6784\u5efa\u3002<\/p>\n\n\n\n

\u4e2d\u79d1\u9662\u56e2\u961f\u5f00\u53d1\u7684\u5168\u606f\u58f0\u5b66\u954a\u5b50\u6280\u672f\u5236\u5907\u7684\u809d\u7ec6\u80de\u7403\u4f53\u5c55\u73b0\u51fa\u4f18\u8d8a\u529f\u80fd\uff0c\u867d\u7814\u7a76\u5c1a\u5904\u65e9\u671f\uff0c\u4f46\u4e3a\u7c7b\u5668\u5b98\u6807\u51c6\u5316\u751f\u4ea7\u63d0\u4f9b\u4e86\u65b0\u601d\u8def\u3002<\/p>\n\n\n\n

3)    3D\u751f\u7269\u6253\u5370<\/h3>\n\n\n\n

3D\u751f\u7269\u6253\u5370\u6280\u672f\u4e3a\u809d\u7c7b\u5668\u5b98\u6784\u5efa\u63d0\u4f9b\u4e86\u9ad8\u6548\u7cbe\u51c6\u7684\u65b0\u65b9\u6cd5\uff0c\u901a\u8fc7\u5fae\u9635\u5217\u6253\u5370\u3001\u6324\u51fa\u5f0f\u6253\u5370\u7b49\u6280\u672f\u5b9e\u73b0\u7ec6\u80de\u548c\u7c7b\u5668\u5b98\u7684\u7a7a\u95f4\u53ef\u63a7\u6c89\u79ef\uff0c\u663e\u8457\u63d0\u5347\u751f\u4ea7\u6548\u7387\u548c\u529f\u80fd\u6027\u3002<\/p>\n\n\n\n

\u8be5\u6280\u672f\u4e0d\u4ec5\u80fd\u6279\u91cf\u5236\u5907\u5747\u4e00\u6027\u9ad8\u7684\u7c7b\u5668\u5b98\u9635\u5217\uff08\u59821\u5206\u949f\u5b8c\u6210\u524d\u80a0\u7ec6\u80de-Matrigel\u6253\u5370\uff09\uff0c\u8fd8\u80fd\u8bbe\u8ba1\u5177\u6709\u4f18\u5316\u6269\u6563\u6027\u80fd\u7684\u590d\u6742\u7ed3\u6784\uff08\u5982200-400\u03bcm\u5b54\u5f84\u809d\u6784\u9020\u4f53\uff09\u3002<\/p>\n\n\n

\n
\"\"
3D\u751f\u7269\u6253\u5370\u809d\u810f\u7c7b\u5668\u5b98<\/figcaption><\/figure>\n<\/div>\n\n\n

\u4f53\u79ef\u751f\u7269\u6253\u5370\u7b49\u975e\u6324\u51fa\u5f0f\u65b9\u6cd5\u66f4\u5b9e\u73b0\u4e86\u5b8c\u6574\u7c7b\u5668\u5b98\u76843D\u652f\u67b6\u5feb\u901f\u5d4c\u5165\uff0820\u79d2\u5b8c\u6210\uff09\uff0c\u4fdd\u6301\u826f\u597d\u6d3b\u6027\u548c\u4ee3\u8c22\u529f\u80fd\u3002<\/p>\n\n\n\n

\u867d\u7136\u4ecd\u9700\u89e3\u51b3\u6253\u5370\u5206\u8fa8\u7387\u3001\u7ec6\u80de\u635f\u4f24\u7b49\u95ee\u9898\uff0c\u751f\u7269\u6253\u5370\u6280\u672f\u6b63\u5728\u63a8\u52a8\u809d\u7c7b\u5668\u5b98\u5411\u66f4\u9ad8\u590d\u6742\u6027\u3001\u751f\u7406\u76f8\u5173\u6027\u548c\u5e94\u7528\u591a\u6837\u6027\u53d1\u5c55\u3002<\/p>\n\n\n\n

4)    \u82af\u7247\u4e0a\u7684\u7c7b\u5668\u5b98<\/h3>\n\n\n\n

Chip<\/a>\u6280\u672f\u901a\u8fc7\u7cbe\u5bc6\u8bbe\u8ba1\u7684\u6d41\u4f53\u901a\u9053\u548c\u52a8\u6001\u57f9\u517b\u73af\u5883\uff0c\u8be5\u6280\u672f\u4e0d\u4ec5\u80fd\u5b9e\u73b0\u7c7b\u5668\u5b98\u7684\u9ad8\u6548\u57f9\u517b\u4e0e\u5206\u5316\uff08\u59823D\u5fae\u67f1\u82af\u7247\u7cfb\u7edf\u4f7f\u809d\u6807\u5fd7\u7269\u8868\u8fbe\u63d0\u5347\uff09\uff0c\u66f4\u901a\u8fc7\u591a\u5668\u5b98\u96c6\u6210\u5f00\u521b\u4e86\u5168\u65b0\u7814\u7a76\u7ef4\u5ea6\u2014\u2014\u5982\u809d\u810f-\u5fc3\u810f\u7c7b\u5668\u5b98\u82af\u7247\u53ef\u8bc4\u4f30\u836f\u7269\u4ee3\u8c22\u6bd2\u6027\uff0c\u809d\u810f-\u80f0\u5c9b\u7cfb\u7edf\u80fd\u6a21\u62df\u751f\u7406\u7ea7\u8461\u8404\u7cd6\u8c03\u63a7\u3002<\/p>\n\n\n

\n
\"\"
\u809d\u810f\u7c7b\u5668\u5b98\u82af\u7247<\/figcaption><\/figure>\n<\/div>\n\n\n

\u8fd9\u4e9b\u82af\u7247\u5e73\u53f0\u7ed3\u5408\u5fae\u6d41\u63a7\u7684\u7cbe\u51c6\u6d41\u4f53\u63a7\u5236\u4f18\u52bf\uff08\u526a\u5207\u5e94\u529b\u3001\u8425\u517b\u8f93\u9001\uff09\u548c\u591a\u5668\u5b98\u4ea4\u4e92\u7279\u70b9\uff0c\u663e\u8457\u63d0\u5347\u4e86\u836f\u7269\u6d4b\u8bd5\u7684\u53ef\u9760\u6027\u548c\u751f\u7406\u76f8\u5173\u6027\uff0c\u4e3a\u590d\u6742\u751f\u7269\u8fc7\u7a0b\u7814\u7a76\u548c\u7cbe\u51c6\u533b\u7597\u63d0\u4f9b\u4e86\u5f3a\u5927\u5de5\u5177\u3002<\/p>\n\n\n\n

3.    \u5de5\u7a0b\u5316\u809d\u810f\u7c7b\u5668\u5b98\u7684\u5e94\u7528<\/h2>\n\n\n\n

1) Disease Modeling<\/h3>\n\n\n\n

\u901a\u8fc7\u57fa\u56e0\u7f16\u8f91\uff08\u5982CRISPR\u4ecb\u5bfc\u7684BAP1\u7a81\u53d8\uff09\u6216\u75c5\u7406\u5fae\u73af\u5883\u8bf1\u5bfc\uff08\u5982\u8102\u80aa\u9178\u66b4\u9732\u6a21\u62df\u8102\u80aa\u809d\uff09\uff0c\u7c7b\u5668\u5b98\u80fd\u7cbe\u51c6\u518d\u73b0\u975e\u9152\u7cbe\u6027\u8102\u80aa\u809d\u3001\u80c6\u7ba1\u764c\u7b49\u75be\u75c5\u7684\u75c5\u7406\u7279\u5f81\u3002<\/p>\n\n\n\n

\u60a3\u8005\u6765\u6e90\u7684iPSCs\u7c7b\u5668\u5b98\u4fdd\u7559\u4e86\u9057\u4f20\u80cc\u666f\uff0c\u5982Alagille\u7efc\u5408\u5f81\u6a21\u578b\u4e2d\u80c6\u7ba1\u53d1\u80b2\u5f02\u5e38\uff0c\u7ecf\u57fa\u56e0\u4fee\u590d\u540e\u53ef\u9006\u8f6c\u8868\u578b\u3002<\/p>\n\n\n\n

\u7ed3\u5408\u5fae\u6d41\u63a7\u82af\u7247\u7684\u52a8\u6001\u57f9\u517b\uff0c\u8be5\u7c7b\u5668\u5b98\u5e73\u53f0\u4e0d\u4ec5\u80fd\u89e3\u6790\u75be\u75c5\u673a\u5236\uff08\u5982c-Myc\u81f4\u764c\u4f5c\u7528\uff09\uff0c\u8fd8\u80fd\u8bc4\u4f30\u57fa\u56e0\u6cbb\u7597\uff08\u5982ASS1\u8f6c\u5bfc\u7ea0\u6b63\u6c28\u4ee3\u8c22\uff09\u548c\u6297\u7ea4\u7ef4\u5316\u836f\u7269\u7684\u6548\u679c\u3002<\/p>\n\n\n\n

2)    \u836f\u7269\u7b5b\u9009<\/h3>\n\n\n\n

\u809d\u7c7b\u5668\u5b98\u6280\u672f\u6b63\u9769\u65b0\u836f\u7269\u6bd2\u6027\u8bc4\u4f30\u4f53\u7cfb\uff0c\u901a\u8fc7\u5fae\u6d41\u63a73D\u6253\u5370\u7b49\u6280\u672f\u5b9e\u73b0\u9ad8\u901a\u91cf\u3001\u6807\u51c6\u5316\u836f\u7269\u7b5b\u9009\uff08\u5982\u5965\u6c99\u5229\u94c2\u809d\u6bd2\u6027\u8282\u5f8b\u7814\u7a76\uff09\u3002<\/p>\n\n\n\n

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3)    \u518d\u751f\u533b\u5b66<\/h3>\n\n\n\n

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