{"id":3873,"date":"2025-03-18T16:30:00","date_gmt":"2025-03-18T08:30:00","guid":{"rendered":"https:\/\/www.dxfluidics.com\/?p=3873"},"modified":"2025-03-18T14:40:04","modified_gmt":"2025-03-18T06:40:04","slug":"%e5%be%ae%e6%b5%81%e6%8e%a7%e7%94%b5%e6%b3%b3%e9%a9%b1%e5%8a%a8%e6%a8%a1%e5%bc%8f","status":"publish","type":"post","link":"https:\/\/www.dxfluidics.com\/en\/technical-support\/information\/3873\/%e5%be%ae%e6%b5%81%e6%8e%a7%e7%94%b5%e6%b3%b3%e9%a9%b1%e5%8a%a8%e6%a8%a1%e5%bc%8f\/","title":{"rendered":"\u5fae\u6d41\u63a7\u7535\u6cf3\u9a71\u52a8\u6a21\u5f0f"},"content":{"rendered":"

\u5fae\u6d41\u63a7\u81ea\u7531\u6d41\u7535\u6cf3\u5177\u6709\u591a\u79cd\u4f18\u52bf\uff0c\u5982\u7f29\u77ed\u6837\u54c1\u505c\u7559\u65f6\u95f4\u3001\u52a0\u5feb\u5206\u79bb\u901f\u5ea6\u3001\u8282\u7ea6\u6210\u672c\u3001\u4fbf\u4e8e\u643a\u5e26\u3001\u53ef\u96c6\u6210\u591a\u4e2a\u64cd\u4f5c\u5355\u5143\u3001\u9ad8\u7eb5\u6a2a\u6bd4\u4fbf\u4e8e\u5feb\u901f\u6563\u70ed\u548c\u8bbe\u7f6e\u9ad8\u7535\u573a\u5f3a\u5ea6\uff0c\u4ee5\u53ca\u5728\u5177\u6709\u5fae\u7c73\u7ea7\u7279\u5f81\u5c3a\u5bf8\u7684\u901a\u9053\u4e2d\u589e\u5f3a\u5c42\u6d41\u72b6\u6001\u3002<\/p>\n\n\n\n

\u751f\u7269\u79d1\u5b66\u5728\u5f88\u5927\u7a0b\u5ea6\u4e0a\u4f9d\u8d56\u4e8e\u86cb\u767d\u8d28\u3001\u80bd\u548c\u6c28\u57fa\u9178\u7684\u9274\u5b9a\u548c\u5206\u79bb\uff0c\u800c\u7535\u6cf3\u8fdb\u6837\u53ef\u5b9e\u73b0\u9ad8\u6548\u5206\u79bb\uff0c\u6837\u54c1\u7a00\u91ca\u7a0b\u5ea6\u4f4e\u3002<\/p>\n\n\n\n

1.    \u7535\u6cf3<\/h2>\n\n\n\n

\u7535\u6cf3\u662f\u6307\u5728\u5916\u90e8\u7535\u573a\u7684\u5f71\u54cd\u4e0b\uff0c\u5e26\u7535\u8868\u9762\u53ca\u5176\u9644\u7740\u7269\u8d28\u76f8\u5bf9\u4e8e\u9759\u6b62\u6db2\u4f53\u7684\u8fd0\u52a8\u3002<\/p>\n\n\n\n

\u8fd9\u79cd\u8fd0\u52a8\u662f\u7531\u7c92\u5b50\u8868\u9762\u7535\u8377\u6240\u4ea7\u751f\u7684\u5e93\u4ed1\u529b\u5f15\u8d77\u7684\u3002<\/p>\n\n\n\n

\u8bb8\u591a\u751f\u7269\u6807\u8bb0\u7269\u7684\u68c0\u6d4b\u90fd\u662f\u4ee5\u5fae\u6d41\u63a7\u7535\u6cf3\u6280\u672f<\/a>\u4e3a\u57fa\u7840\u7684\uff0c\u5982\u6fc0\u5149\u8bf1\u5bfc\u8367\u5149\uff08LIF\uff09\u3001\u7d2b\u5916\u7ebf\uff08UV\uff09\u548c\u8d28\u8c31\uff08MS\uff09\uff0c\u4ee5\u53ca\u6bdb\u7ec6\u7ba1\u533a\u5e26\u7535\u6cf3\uff08CZE\uff09\u3001\u6bdb\u7ec6\u7ba1\u51dd\u80f6\u7535\u6cf3\uff08CGE\uff09\u3001\u7b49\u6e17\u7535\u6cf3\uff08ITP\uff09\u3001\u80f6\u675f\u7535\u52a8\u8272\u8c31\uff08MEKC\uff09\u548c\u7b49\u7535\u805a\u7126\uff08IEF\uff09\u3002<\/p>\n\n\n\n

\u4e0b\u56fe\u63cf\u8ff0\u4e86\u7535\u6cf3\u6a21\u578b\u7684\u4e00\u4e9b\u57fa\u672c\u539f\u7406\u3002<\/p>\n\n\n

\n
\"\"
\u5fae\u7cfb\u7edf\u4e2d\u4f7f\u7528\u7684\u7535\u6cf3\u6a21\u5f0f\uff1a\uff08A\uff09 \u6e38\u79bb\u6eb6\u6db2\u7535\u6cf3\u3002\uff08B\uff09 \u51dd\u80f6\u7535\u6cf3\u3002\uff08C\uff09 \u7b49\u7535\u805a\u7126\u3002\uff08D\uff09 \u80f6\u675f\u7535\u52a8\u8272\u8c31\u6cd5\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n

1)    \u6bdb\u7ec6\u7ba1\u7535\u6cf3<\/h3>\n\n\n\n

\u6709\u522b\u4e8e\u5176\u4ed6\u7535\u6cf3\u6280\u672f\uff0c\u6bdb\u7ec6\u7ba1\u7535\u6cf3\uff08CE\uff09\u91c7\u7528\u7684\u5206\u79bb\u4ecb\u8d28\u662f\u7194\u878d\u77f3\u82f1\u6bdb\u7ec6\u7ba1\u3002<\/p>\n\n\n\n

\u7531\u4e8e\u6bdb\u7ec6\u7ba1\u7535\u6cf3\u5206\u79bb\u6548\u7387\u9ad8\u3001\u8bd5\u5242\u548c\u6837\u54c1\u6d88\u8017\u5c11\u3001\u5206\u6790\u65f6\u95f4\u77ed\uff0c\u56e0\u6b64\u6210\u4e3a\u751f\u7269\u5b66\u548c\u533b\u5b66\u9886\u57df\u4e2d\u4e00\u79cd\u6709\u6548\u7684\u5fae\u5206\u6790\u5e73\u53f0\u3002<\/p>\n\n\n\n

\u6bdb\u7ec6\u7ba1\u7535\u6cf3\u8fd8\u4e0e\u8bb8\u591a\u68c0\u6d4b\u65b9\u6cd5\u517c\u5bb9\uff0c\u5982\u5438\u5149\u5ea6\u6cd5\u3001\u6fc0\u5149\u8bf1\u5bfc\u8367\u5149\u6cd5\u3001\u8d28\u8c31\u6cd5\u548c\u4f0f\u5b89\u6cd5\u3002<\/p>\n\n\n\n

\u5e7f\u6cdb\u7528\u4e8e\u63a7\u5236\u7f13\u51b2\u6db2\u6d41\u91cf\u548c\u5904\u7406\u6837\u54c1\u6eb6\u8d28\uff0c\u5982\u6837\u54c1\u6ce8\u5165\u3001\u5206\u79bb\u3001\u6df7\u5408\u3001\u7a00\u91ca\/\u6d53\u7f29\u548c\u53cd\u5e94\u3002<\/p>\n\n\n\n

CE\u7684\u9ad8\u901a\u91cf\u7279\u6027\u9002\u7528\u4e8e\u5206\u79bb\u5404\u79cd\u5206\u6790\u7269\uff0c\u5178\u578b\u5e94\u7528\u5305\u62ec\u7247\u6bb5\u5927\u5c0f\u4f30\u8ba1\u3001SSCP\u6216RFLP\u5206\u6790\u548cDNA\u6d4b\u5e8f\u3002<\/p>\n\n\n\n

\u4e0d\u8db3\u4e4b\u5904\u5728\u4e8e\u4f7f\u7528CE\u5206\u79bb\u86cb\u767d\u8d28\u4f1a\u51fa\u73b0\u6bdb\u7ec6\u7ba1\u58c1\u5438\u9644\u3001\u6d82\u5c42\u4e0d\u7a33\u5b9a\u4ee5\u53ca\u96be\u4ee5\u63a7\u5236\u7535\u6e17\u6d41\uff08EOF\uff09\u7b49\u60c5\u51b5\u3002<\/p>\n\n\n\n

\u86cb\u767d\u8d28\u5438\u9644\u4f1a\u5bfc\u81f4\u8fc1\u79fb\u6301\u7eed\u65f6\u95f4\u7f29\u77ed\u3001\u68c0\u6d4b\u54cd\u5e94\u964d\u4f4e\u3001\u91cd\u73b0\u6027\u5dee\u3001\u5206\u8fa8\u7387\u964d\u4f4e\u548c\u5206\u79bb\u6548\u7387\u4e0b\u964d\u3002<\/p>\n\n\n\n

\u4e0d\u53ef\u518d\u73b0\u7684\u7535\u6e17\u6d41\uff08EOF\uff09\u53ef\u80fd\u4f1a\u5f71\u54cd\u5206\u79bb\u86cb\u767d\u8d28\u7684\u5206\u8fa8\u7387\u548c\u53ef\u91cd\u590d\u6027\u3002<\/p>\n\n\n\n

\u4e3a\u4e86\u51cf\u5c11\u86cb\u767d\u8d28\u7684\u975e\u7279\u5f02\u6027\u9644\u7740\uff0c\u9700\u8981\u8fdb\u884c\u8868\u9762\u5904\u7406<\/a>.<\/p>\n\n\n\n

\u7531\u4e8e\u6bdb\u7ec6\u7ba1\u7535\u6cf3\u6ca1\u6709\u79fb\u52a8\u90e8\u4ef6\u3001\u65e0\u8109\u51b2\u6cf5\u3001\u53ef\u5feb\u901f\u5206\u79bb\u5c11\u91cf\u6837\u54c1\uff0cCE\u7ecf\u5e38\u88ab\u7528\u4e8e\u6d89\u53caDNA\u7684\u751f\u7269\u5e94\u7528\u4e2d\u3002<\/p>\n\n\n\n

\u7528\u4e8e\u5206\u79bb\u548c\u68c0\u6d4bDNA\/RNA\u7684\u5e38\u89c1CE\u82af\u7247\u5e03\u5c40\u5982\u56fe\u6240\u793a\u3002<\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

\u6bdb\u7ec6\u7ba1\u7535\u6cf3\u6280\u672f\u4e0d\u4ec5\u53ef\u7528\u4e8eDNA\u5206\u79bb\uff0c\u8fd8\u53ef\u7528\u4e8eRNA\u5206\u79bb\u3002<\/p>\n\n\n

\n
\"\"
\u86cb\u767d\u8d28\u4fc3\u8fdb\u7684\u4eb2\u548c\u6bdb\u7ec6\u7ba1\u7535\u6cf3 \uff08ProFACE\uff09 \u7528\u4e8e\u68c0\u6d4b miRNA<\/figcaption><\/figure>\n<\/div>\n\n\n

2)    \u51dd\u80f6\u7535\u6cf3<\/h3>\n\n\n\n

\u51dd\u80f6\u7535\u6cf3\u7528\u4e8e\u5316\u5408\u7269\u5206\u79bb\uff0c\u53ef\u91c7\u7528\u6bdb\u7ec6\u7ba1\u51dd\u80f6\u6216\u677f\u5757\u51dd\u80f6\u5f62\u5f0f\uff0c\u4e5f\u53ef\u91c7\u7528\u5404\u79cd\u5f62\u5f0f\u7684\u4eb2\u548c\u5c42\u6790\u3002<\/p>\n\n\n\n

\u5728\u51dd\u80f6\u7535\u6cf3\u8fc7\u7a0b\u4e2d\uff0c\u7535\u573a<\/a>\u4f1a\u63a8\u52a8\u5e26\u6709\u5fae\u5b54\u7684\u51dd\u80f6\u57fa\u8d28\uff0c\u4ece\u800c\u5206\u79bb\u5206\u5b50\u3002\u51dd\u80f6\u7535\u6cf3\u53ef\u4ee5\u51cf\u5c11\u6269\u6563\uff0c\u6269\u5927\u5206\u79bb\u533a\u57df\uff0c\u56e0\u4e3a\u51dd\u80f6\u57fa\u8d28\u4f1a\u5438\u9644\u5728\u5fae\u901a\u9053\u58c1\u4e0a\uff0c\u4e2d\u548c\u8868\u9762\u7535\u8377\u3002<\/p>\n\n\n\n

\u51dd\u80f6\u7535\u6cf3\u5e38\u7528\u7684\u4e24\u79cd\u4ecb\u8d28\u662f\u805a\u4e19\u70ef\u9170\u80fa\u548c\u743c\u8102\u7cd6\u3002\u743c\u8102\u7cd6\u5f62\u6210\u7684\u7269\u7406\u51dd\u80f6\u901a\u5e38\u7528\u4e8e\u5206\u79bb\u8f83\u957f\u7684DNA\uff0c\u800c\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u662f\u5316\u5b66\u4ea4\u8054\u7684\uff0c\u7528\u4e8e\u5206\u79bb\u8f83\u77ed\u7684\u53cc\u94fe\u548c\u5355\u94feDNA\u3002<\/p>\n\n\n\n

DNA\u5206\u5b50\u5728\u51dd\u80f6\u7535\u6cf3\u8fc7\u7a0b\u4e2d\u7684\u8fc1\u79fb\u7387\u53d7\u591a\u79cd\u56e0\u7d20\u5f71\u54cd\u3002<\/p>\n\n\n\n

\u4f8b\u5982\uff0c\u5f53DNA\u5206\u5b50\u5c0f\u4e8e\u51dd\u80f6\u5b54\u5f84\u65f6\uff0c\u4f1a\u53d1\u751f\u5965\u683c\u65af\u901a\u7b5b\u5206\uff08\u56feA\uff09\u3002<\/p>\n\n\n\n

\u5f53DNA\u7684\u5927\u5c0f\u63a5\u8fd1\uff08\u56feB\uff09\u6216\u8d85\u8fc7\u51dd\u80f6\u5b54\u5f84\uff08\u56feC\uff09\u65f6\uff0c\u5b83\u5c31\u4f1a\u5728\u51dd\u80f6\u4e2d\u53cd\u8f6c\u6216\u62c9\u4f38\u53d8\u5f62\u3002<\/p>\n\n\n\n

\u5229\u7528\u8fd9\u4e9b\u4e0d\u540c\u7684\u7b5b\u5206\u6a21\u5f0f\u53ef\u4f18\u5316\u57fa\u4e8e\u51dd\u80f6\u7684\u5206\u79bb\uff0c\u4ece\u800c\u5206\u8fa8\u7ed9\u5b9a\u6837\u54c1\u4e2d\u7684\u7279\u5b9a\u7c92\u5ea6\u8303\u56f4\u3002<\/p>\n\n\n

\n
\"\"
\u5229\u7528\u51dd\u80f6\u7535\u6cf3\u7684 DNA \u5206\u79bb\u8fc7\u7a0b\u3002\uff08A\uff09 Rg\/a \u226a 1.\uff08B\uff09 Rg\/a \u2248 1 \uff08C\uff09 Rg\/a \u226b 1.<\/figcaption><\/figure>\n<\/div>\n\n\n

\u4e00\u822c\u6765\u8bf4\uff0c\u51dd\u80f6\u7535\u6cf3\u7684\u4f18\u70b9\u5728\u4e8e\u80fd\u5c06\u6240\u6709\u8fc7\u7a0b\u6574\u5408\u5230\u4e00\u4e2a\u82af\u7247<\/a>\u4e0a\uff0c\u5305\u62ec\u7ec6\u80de\u5206\u62e3\u3001\u7ec6\u80de\u88c2\u89e3\u3001\u63d0\u53d6\u76f8\u5173\u7269\u8d28\uff08\u5982DNA\uff09\u3001\u7eaf\u5316\u3001\u5206\u79bb\u548c\u5206\u6790DNA\u3002<\/p>\n\n\n\n

\u5fae\u5c3a\u5ea6\u901a\u9053\u4e2d\u7684\u51dd\u80f6\u533a\u751a\u81f3\u6709\u52a9\u4e8e\u4ece\u566c\u83cc\u4f53\u4ee5\u53caMCF-7\u7ec6\u80de\u548cSKBR3\u7ec6\u80de\u4e2d\u63d0\u53d6DNA\u3002<\/p>\n\n\n

\n
\"\"<\/figure>\n<\/div>\n\n\n

\u7f3a\u9677\u662f\uff1a\u5728\u5206\u79bb\u8fc7\u7a0b\u4e2d\uff0c\u5206\u79bb\u53c2\u6570\u65e0\u6cd5\u6539\u53d8\u6216\u4f18\u5316\uff1b\u660e\u663e\u7684\u526a\u5207\u529b\u6548\u5e94\u4f1a\u5f71\u54cd\u5206\u6790\u7269\u7684\u5b8c\u6574\u6027\u548c\u529f\u80fd\uff1b\u6837\u54c1\u6d17\u8131\u548c\u540e\u5904\u7406\u53ef\u80fd\u5177\u6709\u6311\u6218\u6027\uff0c\u751a\u81f3\u53ef\u80fd\u9700\u8981\u4f7f\u7528\u6709\u6bd2\u6216\u8bf1\u53d8\u8bd5\u5242\uff1b\u8fc7\u7a0b\u662f\u534a\u5b9a\u91cf\u7684\uff1b\u5206\u6790\u8fc7\u7a0b\u53ef\u80fd\u5f88\u8017\u65f6\u3002<\/p>\n\n\n\n

3)    \u81ea\u7531\u6d41\u7535\u6cf3\uff08FFE\uff09<\/h3>\n\n\n\n

\u81ea\u7531\u6d41\u7535\u6cf3\uff08FFE\uff09\u53c8\u79f0\u65e0\u8f7d\u4f53\u7535\u6cf3\uff0c\u662f\u4e00\u79cd\u65e0\u57fa\u8d28\u3001\u9ad8\u538b\u7535\u6cf3\u5206\u79bb\u6280\u672f\u3002<\/p>\n\n\n\n

\u5728\u6d41\u4f53\u52a8\u529b\u6cf5\u9001\u6db2\u4f53\u7cfb\u7edf\u4e2d\uff0c\u6837\u54c1\u88ab\u5f15\u5165\u8f7d\u4f53\u7535\u89e3\u8d28\u4e2d\uff0c\u5e76\u5728\u5782\u76f4\u4e8e\u6d41\u4f53\u7684\u7535\u573a\u4f5c\u7528\u4e0b\u4ee5\u5c42\u6d41\u72b6\u6001\u6d41\u7ecf\u901a\u9053\u3002<\/p>\n\n\n\n

\u76ee\u524d\u5df2\u5f00\u53d1\u51fa\u5305\u62ec\u81ea\u7531\u6d41\u52a8\u533a\u7535\u6cf3\u548c\u81ea\u7531\u6d41\u52a8\u7b49\u7535\u805a\u7126\u5728\u5185\u7684\u591a\u79cd\u6280\u672f\uff0c\u53ef\u7528\u4e8e\u5206\u79bbDNA\u3001\u7ec6\u80de\u3001\u86cb\u767d\u8d28\u5f02\u6784\u4f53\u3001\u80bd\u548c\u7eb3\u7c73\u9897\u7c92\u7b49\u591a\u529f\u80fd\u7269\u8d28\u3002<\/p>\n\n\n

\n
\"\"
m-FFE \u5c0f\u5de5\u5177\u53ca\u5176\u5206\u79bb\u673a\u5236\u7684\u56fe\u793a\u3002\uff08I \u548c III\uff09\u5e94\u7528\u81ea\u7531\u6d41\u52a8\u533a\u7535\u6cf3\u3002\uff08II \u548c IV\uff09\u81ea\u7531\u6d41\u52a8\u7b49\u7535\u805a\u7126\u5e94\u7528\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n

FFE\u7684\u4e3b\u8981\u4f18\u70b9\u662f\u53ef\u4ee5\u5feb\u901f\u3001\u6e29\u548c\u5730\u5206\u79bb\u6eb6\u89e3\u5728\u6db2\u4f53\u6eb6\u5242\u4e2d\u7684\u6837\u54c1\uff0c\u65e0\u9700\u57fa\u8d28\u3002<\/p>\n\n\n\n

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