{"id":1111,"date":"2023-05-12T13:04:52","date_gmt":"2023-05-12T05:04:52","guid":{"rendered":"https:\/\/www.dxfluidics.com\/?p=1111"},"modified":"2025-09-09T23:31:11","modified_gmt":"2025-09-09T15:31:11","slug":"%e8%a1%a8%e9%9d%a2%e6%94%b9%e6%80%a7-%e4%bf%ae%e9%a5%b0-peg%e4%bf%ae%e9%a5%b0","status":"publish","type":"post","link":"https:\/\/www.dxfluidics.com\/en\/technical-support\/process-introduction\/1111\/%e8%a1%a8%e9%9d%a2%e6%94%b9%e6%80%a7-%e4%bf%ae%e9%a5%b0-peg%e4%bf%ae%e9%a5%b0\/","title":{"rendered":"Surface modification\/modification - PEG modification"},"content":{"rendered":"

Biochip surface PEG modification is a surface modification method that introduces polyethylene glycol (PEG) functional groups on the surface of a biochip.<\/p>\n\n\n\n

PEG modifications can be used to alter the chemical and physical properties of the chip surface to achieve specific application requirements.PEG modifications are typically performed by chemically binding PEG molecules to the chip surface to form a stable PEG layer.<\/p>\n\n\n\n

Below are the steps outlined for PEG modification on the surface of a biochip:<\/strong><\/p>\n\n\n\n

    \n
  1. Surface activation: The surface of a biochip usually needs to be activated to increase the reactivity and hydrophilicity of the surface. Common methods of activation include plasma treatment, UV irradiation, or the use of chemically activated agents. The purpose of this step is to remove impurities from the surface and provide a suitable base for subsequent PEG modifications.<\/li>\n\n\n\n
  2. Surface functionalization: After surface activation, appropriate functional groups need to be introduced on the chip surface so that the PEG molecules can react with them. Common surface functionalization methods include silylation, carboxylation, amination, and so on. The selection of the appropriate functionalization method depends on the chemical properties of the chip surface and the desired purpose of PEG modification.<\/li>\n\n\n\n
  3. PEG modification: PEG molecules usually have reactive functional groups such as hydroxyl, amine or anhydride groups. Through chemical reaction, the PEG molecules are combined with the surface of the surface-functionalized chip to form a PEG modification layer. Commonly used PEG modification methods include the reaction of hydroxyl groups with isocyanates, the reaction of amine groups with anhydrides, etc. PEG modification can form a homogeneous layer covering the entire surface, or it can selectively modify specific areas.<\/li>\n\n\n\n
  4. Elution and closure: After completing PEG modification, unreacted compounds need to be eluted to avoid interference with subsequent experiments. Commonly used elution methods include solvent elution, centrifugal elution or surfactant elution. Finally, the remaining reactive sites can be closed by reacting with nucleophilic reagents or reactants to prevent non-specific adsorption.<\/li>\n<\/ol>\n\n\n\n

    The role of PEG modifications on the surface of biochips<\/strong><\/h2>\n\n\n\n

    1, anti-contamination: PEG-modified layer has excellent hydrophilicity and anti-protein adsorption, which can inhibit non-specific adsorption and cell attachment, thus inhibiting non-specific adsorption and cell attachment, thus reducing background noise and improving the specificity of detection. This is essential for the sensitivity and reliability of the biochip.<\/p>\n\n\n\n

    2, Biocompatibility: The PEG-modified layer has good biocompatibility and can reduce non-specific interactions between the biochip and the biological samples. It reduces the recognition and adhesion of cells and proteins, which reduces non-specific interference and improves the specific interaction between the biological sample and the chip.<\/p>\n\n\n\n

    3\u3001Hydrophilicity regulation: The PEG modified layer can regulate the hydrophilicity of the surface of the biochip so that it has proper wettability and droplet expansion. This is crucial for the precise control of biological droplets and the accuracy of sample analysis.<\/p>\n\n\n\n

    4, long-term stability: PEG modification layer can provide long-term stability of the surface of the biochip and reduce the aging and destruction of the modification layer to ensure long-term service life and reliable performance.<\/p>\n\n\n\n

    Overall, PEG modification on the surface of biochips achieves anti-pollution, biocompatibility, hydrophilicity modulation and long-term stability by introducing a PEG layer. This modification method has a wide application potential in the fields of biomolecular analysis, cell research, biosensing and drug screening on biochips.<\/p>\n\n\n\n

    Dxfluidics Processing Capabilities<\/h2>\n\n\n\n
    \"\"<\/figure>\n\n\n\n

    DXFLUIDICS<\/h2>\n\n\n\n

    An innovative company focusing on microfluidic chips and biochips, etc., is able to provide customized surface modification\/modification services for microfluidic chips and biochips with the help of the company's new material surface coating processing technology. Our coating processing technology can enhance the surface properties of chips, improve their durability, corrosion resistance and biocompatibility, and realize specific functions, such as enhanced signal transmission, antimicrobial and cell adhesion control. By partnering with us, customers can gain access to advanced coating solutions in the field of microfluidic chips and biochips, opening up a wider range of possibilities for their research and applications.<\/p>\n\n\n\n

    For more details, please consult the customer service: Zhou Manager 17751163890 (with WeChat)<\/p>","protected":false},"excerpt":{"rendered":"

    \u751f\u7269\u82af\u7247\u8868\u9762PEG\u4fee\u9970\u662f\u6307\u5728\u751f\u7269\u82af\u7247\u8868\u9762\u5f15\u5165\u805a\u4e59\u4e8c\u9187\uff08polyethylene glycol\uff0c\u7b80\u79f0PEG\uff09\u5b98\u80fd […]<\/p>\n","protected":false},"author":1,"featured_media":1113,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_crdt_document":"","footnotes":""},"categories":[89],"tags":[],"class_list":["post-1111","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-process-introduction"],"yoast_head":"\n\u8868\u9762\u6539\u6027\/\u4fee\u9970\u2014\u2014PEG\u4fee\u9970 - \u9876\u65ed\u5fae\u63a7<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.dxfluidics.com\/en\/technical-support\/process-introduction\/1111\/\u8868\u9762\u6539\u6027-\u4fee\u9970-peg\u4fee\u9970\/\" 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